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|Title:||Treatment with anti-transforming growth factor β antibodies influences an altered pattern of cytokines gene expression in injured rat liver|
|Abstract:||The role of transforming growth factor β1 (TGF-β1) in mediating hepatic inflammation and regeneration after acute liver injury is beginning to be elucidated, yet its in vivo effect on the gene expression of the major pro-inflammatory and antiinflammatory cytokines produced during that process is unknown. Our previous experiments demonstrated that anti-TGF-β-treated animals presented profound histological changes as compared with control animals. Therefore, our hypothesis was that by blocking in vivo TGF-β1 action, with polyclonal anti-TGF-β antibodies, we could monitor by RT-PCR significative alterations on the gene expression of IL-1β, IL-6, TGF-β, TNF-α, IL-4 and IL-10 in liver-regenerated rats after administration of a single CCl4 dosing. Accordingly, we here report a completely different pattern of cytokines gene expression amidst those groups of rats. Pro- inflammatory cytokines gene expression in control animals showed a clear-cut pattern peaking at 1-2 days postinjury and declining thereafter. Interestingly, IL-6 was present in the control animals only between 12 and 24 h after CCl4 dosing. In the experimental animals, TGF-β1 was mainly increased at 4 and 6 days, while IL-6 mRNA was completely absent. IL-lβ mRNA expression was also altered in the experimental rats, albeit TNF-α was nearly unaffected. IL-4 was fully absent in control rats, but remarkably expressed in experimental animals throughout the study. IL-10 was also more expressed in experimental animals.|
|Appears in Collections:||Producción científica UdeG (prueba)|
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