Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.12104/68306
Title: Toll-like receptor-1 and receptor-2 and beta-defensin in postcholecystectomy bile duct injury
Author: Miranda-Diaz, A.G.
Hermosillo-Sandoval, J.M.
Villanueva-Perez, M.A.
Roman-Pintos, L.M.
Garcia-Iglesias, T.
Rodriguez-Carrizalez, A.D.
Cardona-Munoz, E.G.
Issue Date: 2015
Abstract: Postcholecystectomy bile duct injuries (BDI) produce hepatic cholestasis and cause infection of the biliary tract. The biliary cells participate in secreting cytokines and in expression of immune response receptors. Toll-like receptors (TLRs) conduct signalling and activate the innate and adaptive inflammatory response. The objective was to determine the serum levels of TLR-2 and the expression of TLR-1 and TLR-2 and β-defensin in liver biopsies of postcholecystectomy BDI patients. A transverse, analytical study with 2 groups was done. One group included healthy volunteers (control group) and other included 25 postcholecystectomy BDI patients with complete biliary obstruction. Using the Enzyme-linked Immunosorbent Assay (ELISA) technique, serum levels of TLR-2 were determined, and with immunofluorescence the morphologic analysis of TLR-1 and TLR-2 and β-defensin in liver biopsies of postcholecystectomy BDI patients was performed. The average TLR-2 serum level in the control group was 0.0 pg/mL and in the BDI group, 0.023±0.0045 pg/mL (P<0.0001, bilateral Mann Whitney U). Immunofluorescence was used to determine the expression in liver biopsies, blood vessels, bile ducts, and hepatic parenchyma where 12 hepatic biopsies were positive for TLR-1 with average of 3213057.74±1071019.25 μm2; and 7 biopsies were positive for β-defensin with an average of 730364.33±210838.02 μm2; and 6 biopsies positive for TLR-2, obtaining an average of 3354364.24±838591.06 μm2. In conclusion, TLR-1 and TLR-2 and β-defensin play an important role in the innate antimicrobial defense of the hepatobiliary system. © 2015 Alejandra Guillermina Miranda-Díaz et al.
URI: http://hdl.handle.net/20.500.12104/68306
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