Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.12104/67616
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dc.contributor.authorVazquez-Del Mercado, M.
dc.contributor.authorSanchez-Orozco, L.V.
dc.contributor.authorPauley, B.A.
dc.contributor.authorChan, J.Y.F.
dc.contributor.authorChan, E.K.L.
dc.contributor.authorPanduro-Cerda, Arturo
dc.contributor.authorMaldonado-Gonzalez, M.
dc.contributor.authorJimenez-Luevanos, M.A.
dc.contributor.authorMartin-Marquez, B.T.
dc.contributor.authorPalafox-Sanchez, C.A.
dc.contributor.authorDavalos-Rodriguez, I.P.
dc.contributor.authorSalazar-Paramo, M.
dc.contributor.authorGonzalez-Lopez, L.
dc.contributor.authorGamez-Nava, J.I.
dc.contributor.authorSatoh, M.
dc.date.accessioned2015-11-19T18:52:20Z-
dc.date.available2015-11-19T18:52:20Z-
dc.date.issued2010
dc.identifier.urihttp://hdl.handle.net/20.500.12104/67616-
dc.description.abstractObjectives Chronic liver diseases caused by hepatitis B (HBV) or C virus (HCV) are common worldwide. Despite reports on autoimmunity in viral hepatitis, studies on autoantibodies associated with systemic rheumatic diseases are inconsistent. Testing of a small number of selected autoantibody specificities using ELISA appears to be one reason for inconsistency. Sera from patients with viral hepatitis were tested by immunoprecipitation that will allow unbiased screening of autoantibodies found in systemic rheumatic diseases. Methods Ninety Mexican patients (37 male, 53 female, 26 HBV, 6 HBV+HCV, 58 HCV) with chronic viral hepatitis, confirmed by nested or RT-nested-PCR, HBsAg and anti-HCV antibodies, were studied. Autoantibodies were tested by immunofluorescence, immunoprecipitation and ELISA. Specificities were verified using reference sera. Results Antinuclear antibodies were found in 38% HBV, 17% HBV+HCV, and 28% in HCV. Autontibodies to Argonaute (Ago2, Su antigen), a microRNA binding protein that plays a key role in RNA-induced silencing complex (RISC), was found in 5% (4164) of HCV or HBV+HCV coinfected patients but not in HBV (0126). Anti-Ago2/Su was found in 112 of I-IFN-treated case vs. 3162 in cases without 1-IFN. HCVdid not have other lupus autoantibodies whereas 19% (5126) of HBV had anti-UlRNP+Ku, Ro+La, RNA polymerase II, or possible U5snRNPs. Conclusion Lupus autoantibodies were uncommon in HCV except anti-Ago2/Su. HCV and I-IFN have many ways to affect TLR signaling, miRNA and miRNA binding protein Ago2/Su. To understand the mechanism of specific targeting of Ago2 in HCV may provide a clue to understand the mechanism of specific autoantibody production. © 2010 Copyright CLINICAL AND EXPERIMENTAL RHEUMATOLOGY.
dc.titleAutoantibodies to a miRNA-binding protein Argonaute2 (Su antigen) in patients with hepatitis C virus infection
dc.typeArticle
dc.relation.ispartofjournalClinical and Experimental Rheumatology
dc.relation.ispartofvolume28
dc.relation.ispartofissue6
dc.relation.ispartofpage842
dc.relation.ispartofpage848
dc.subject.keywordArgonaute2; Autoantibodies; Hepatitis B; Hepatitis C; MicroRNA; Viral hepatitis
dc.contributor.affiliationVázquez-Del Mercado, M., Instituto de Investigación en Reumatología, Del Sistema Músculo Esquelético (HRSME), Edificio P, Sierra Mojada 950, Puerta 7, Planta baja, ala oriente, CP 44340, Mexico, Departamento de Reumatologia, Hospital Civil Dr. Juan i Menchaca, Guadalajara, Mexico; Sánchez-Orozco, L.V., Instituto de Biologia Molecular en Medicina Departamento de Biologia Molecular y Genómica, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Mexico; Pauley, B.A., Department of Oral Biology, United States; Chan, J.Y.F., Division of Rheumatology and Clinical Immunology, Department of Medicine, United States; Chan, E.K.L., Department of Oral Biology, United States; Panduro, A., Servicio de Biología Molecular en Medicina, O.P.D. Hospital Civil Fray Antonio Alcalde, Guadalajara, Jalisco, Mexico; Maldonado-González, M., Servicio de Biología Molecular en Medicina, O.P.D. Hospital Civil Fray Antonio Alcalde, Guadalajara, Jalisco, Mexico; Jiménez-Luévanos, M.A., Hospital Valentin Gomez Farias, ISSSTE, Guadalajara, Jalisco, Mexico; Martín-Márquez, B.T., Instituto de Investigación en Reumatología, Del Sistema Músculo Esquelético (HRSME), Edificio P, Sierra Mojada 950, Puerta 7, Planta baja, ala oriente, CP 44340, Mexico; Palafox-Sánchez, C.A., Instituto de Investigación en Reumatología, Del Sistema Músculo Esquelético (HRSME), Edificio P, Sierra Mojada 950, Puerta 7, Planta baja, ala oriente, CP 44340, Mexico; Dávalos-Rodríguez, I.P., División de Genética, CIBO, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico; Salazar-Páramo, M., División de Investigación, Hospital de Especialidades, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico; González-López, L., Servicio de Reumatologia, Hospital Regional 110, IMSS, Guadalajara, Jalisco, Mexico; Gámez-Nava, J.I., Unidad de Investigación en Epidemiología Clínica, IMSS, Hospital de Especialidades Centro Médico Nacional de Occidente, Guadalajara, Jalisco, Mexico; Satoh, M., Division of Rheumatology and Clinical Immunology, Department of Medicine, United States, Department of Pathology Immunology, Laboratory Medicine University of Florida, Gainesville, FL, United States, Division of Rheumatology and Clinical Immunology, Dept. of Medicine, University of Florida, 1600 SW Archer Road, Gainesville, FL 32601-0424, United States
dc.contributor.affiliationPanduro-Cerda, Arturo., Universidad de Guadalajara
dc.subject.headingIndex Medicus;Adolescent;Adult;Aged;Antibody Specificity;Argonaute Proteins;Autoantibodies/im [Immunology];Child;Eukaryotic Initiation Factor-2/im [Immunology];Female;Hepacivirus/im [Immunology];Hepacivirus/ph [Physiology];Hepatitis B/bl [Blood];Hepatitis B/im [Immunology];Hepatitis B Surface Antigens/bl [Blood];Hepatitis C/bl [Blood];Hepatitis C/im [Immunology];Hepatitis C Antibodies/bl [Blood];Humans;Immunoprecipitation/mt [Methods];Interferon Type I/me [Metabolism];Male;MicroRNAs/me [Metabolism];Middle Aged;Toll-Like Receptors/me [Metabolism];Young Adult
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dc.identifier.urlhttp://ovidsp.ovid.com/ovidweb.cgi?T=JS&CSC=Y&NEWS=N&PAGE=fulltext&D=med5&AN=21122261
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