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Title: Production of fructanase by a wild strain of Saccharomyces cerevisiae on tequila agave fructan
Author: Corona-Gonzalez, R.I.
Pelayo-Ortiz, C.
Jacques, G.
Guatemala, G.
Arriola, E.
Arias, J.A.
Toriz, G.
Issue Date: 2014
Abstract: A new wild strain of Saccharomyces cerevisiae (CF3) isolated from tequila must was evaluated for production of fructanase on Agave tequilana Weber fructan (FT). Fructanase activity (F) was assessed by a 33 factorial design (substrate, temperature and pH). High enzymatic activity (31.1 U/ml) was found at 30 °C, pH 5, using FT (10 g/l) as substrate. The effect of initial substrate concentration on F (FT0, 5.7–66 g/l) was studied and it was found that F was highest (44.8 U/ml) at FT0 25 g/l. A 22 factorial experimental design with five central points was utilized to study the effect of stirring and aeration on fructanase activity; stirring exhibited a stronger effect on F. The ratio fructanase to invertase (F/S) was 0.57, which confirms that the enzymes are fructanase. Crude fructanase reached high substrate hydrolysis (48 wt%) in 10 h. It is shown that S.cerevisiae CF3 was able to produce large amounts of fructanase by growing it on fructan from A. tequilana. © 2014 Springer International Publishing Switzerland
Appears in Collections:Producción científica UdeG (prueba)

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