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Title: Optical tweezers experiments for fibroblast cell growth stimulation
Author: Avila, R.
Medina-Villalobos, N.
Tamariz, E.
Chiu, R.
Lopez-Marin, L.M.
Acosta, A.
Castano, V.
Issue Date: 2014
Abstract: Optical tweezers constitute an increasingly used tool for the study of biomechanical properties of cells. Here we report experiments for the projection induction of NIH3T3 fibroblast cells, using a single-trap optical tweezers. The system is based on a 1064-nm, 50mW infrared gaussian laser beam, a 100x microscope objective with 1.25 numerical aperture and a temperature-controlled warming plate to maintain cell viability. Eighteen cells were exposed to the focussed laser beam in different cell zones and another 18 cells were observed without laser stimulation as a control population. The results show that the probability of lamelipodia growth increases on exposed cells by a factor 1.5. © 2014 SPIE.
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