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Title: Nitric oxide involvement in regulating the dopamine transport in the striatal region of rat brain
Author: Chaparro-Huerta, V.
Beas-Zárate, Carlos
Guerrero, M.U.
Feria-Velasco, A.
Issue Date: 1997
Abstract: Spontaneous [3H]dopamine ([3H]DA) overflow was measured from striatal slices in the presence of different glutamate (Glu) receptor agonists such as N-methyl-D-aspartate (NMDA), kainate (KA0 and quisqualate (QA) and their corresponding antagonists, Dizocilpine maleate ((MK-801), D-γ-glutamyl-aminomethanesulfonic acid (GAMS) and 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) respectively. [3H]DA uptake and release in the presence of L-Arginine (L-Arg) and N(G)-nitro-arginine (L-N-Arg), an inhibitor of nitric oxide (NO) synthesis were also evaluated. L-N-Arg alone or combined with L-Arg significantly reduced [3H]DA uptake at 10 and 100 μM from 33% to 44% from striatal slices. Whereas, in brain synaptosomal fractions L-Arg induced a biphasic effect on that [3H]DA uptake in a dose dependent manner, and L-N-Arg showed an absolute inhibition in 80-90% of this [3H]DA uptake at 1-500 μM. The amino acids, lysine, valine and histidine (100 μM) had a little effect inhibitory on [3H]DA uptake from synaptosomal fractions. Glu agonists, NMDA (10 μM) and KA (10 μM) importantly increased the spontaneous [3H]DA overflow, which was blocked by MK-801 (10 μM)and GAMS (10 μM), respectively. QA had no effect on [3H]DA release. L-Aug (10-200 μM) potentiated the spontaneous [3H]DA overflow in a dose dependent fashion from striatal slices, being reverted by 10 μM L-N-Arg alone or in combination with all other compounds; whereas, lysine, histidine and valine did not modify that spontaneous [3H]DA overflow. Results support the hypothesis related to the participation of NO on DA transport possibly synthesized at the dopaminergic (DAergic) terminals in the striatum; also that L-Arg concentration may determine alternative mechanisms to regulate the DAergic activity at the striatum.
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