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|Title:||Functional disorders of F0F1-ATPase in submitochondrial particles obtained from platelets of patients with a diagnosis of probable Alzheimer's disease [Alteración funcional de la F0F1-ATPasa en partículas submitocondriales obtenidas de plaquetas de pacientes con diagnóstico de enfermedad de Alzheimer probable]|
|Abstract:||Introduction. Recent studies indicate that decreased energy generation by mitochondria is a feature that is common across neurodegenerative diseases. Patients and methods. In order to obtain direct evidence that mitochondrial functioning is altered, we measured the hydrolytic activity of F 0F1-ATPase and its capacity to generate a stable proton gradient in submitochondrial particles in 29 patients diagnosed with probable Alzheimer's disease (AD). Submitochondrial particles were obtained from platelets of patients with a diagnosis of probable AD and from clinically healthy controls. Results. Data revealed that the hydrolytic activity of F 0F1-ATPase increases significantly in patients with probable AD (41.7 ± 4.3 nmol PO4 min-1 [mg protein]-1, n = 29) as compared to the control subjects (29.1 ± 1.9 nmol PO4 min-1 [mg protein]-1, n = 29). It is important to note that, in the male population with probable AD, we found that hydrolytic activity of F0F1-ATPase increased as cerebral deterioration progressed. We also detected a lower pH gradient in the submitochondrial particles of patients with probable AD (0.28 ± 0.08 pH units, n = 25) as compared to the controls (0.5 ± 0.1 pH units, n = 20). Conclusions. Overall, these data point to an alteration in the functioning of the enzyme. © 2005, Revista de Neurología.|
|Appears in Collections:||Producción científica UdeG (prueba)|
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