Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.12104/63049
Title: Additive effect of ethanol and HCV subgenomic replicon expression on COX-2 protein levels and activity
Author: Trujillo-Murillo, K.
Alvarez-Martinez, O.
Garza-Rodriguez, L.
Martinez-Rodriguez, H.
Bosques-Padilla, F.
Ramos-Jimenez, J.
Barrera-Saldana, H.
Rincon-Sanchez, A.R.
Rivas-Estilla, A.M.
Issue Date: 2007
Abstract: The mechanisms by which alcohol exacerbates liver injury in patients with hepatitis C are unknown. We used the hepatitis C virus (HCV) subgenomic replicon cell system to evaluate the effect of ethanol on HCV replication and viral protein synthesis. Our results demonstrate that alcohol stimulates HCV replicon expression at both HCV-RNA and protein levels. Furthermore, we observed that ethanol treatment showed an additive effect in cyclooxygenase-2 (COX-2) protein expression and activity already induced by HCV viral proteins, and in turn increased HCV viral expression. Our results suggest that COX-2 activity is involved in ethanol-induced HCV-RNA and NS5A protein expression, because acetylsalicylic acid (ASA), a COX-1/2 inhibitor, blocked this induction and downregulated COX-2 protein expression and activity. Therefore, we suggest that ethanol increases HCV replication expression, at least in part, by upregulating a key cellular regulator of oxidative stress pathway known as COX-2 or its products. © 2007 The Authors.
URI: http://hdl.handle.net/20.500.12104/63049
Appears in Collections:Producción científica UdeG (prueba)

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