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Title: Abnormalities in intracellular processing and expression of interferon-γ receptor in adherent cells from lepromatous leprosy patients
Author: Guerrero-Velazquez, C.
Lopez-Roa, R.I.
Delgado-Rizo, V.
Guillen-Vargas, C.M.
Montoya-Buelna, M.
Fafutis-Morris, M.
Issue Date: 2010
Abstract: Peripheral blood mononuclear cells in lepromatous leprosy (LL) patients produce low levels of interferon-γ (IFN-γ) and interleukin-12 (IL-12), and these cells exhibit partial or complete defi ciency in the IL-12 receptor. The behavior of the IFN-γ receptor (IFN-γR) has not been described in cells from people with leprosy. We found higher levels of mRNA for IFN-γR1 and IFN-γR2 in adherent cells stimulated with IFN-γ and Mycobacterium leprae membrane proteins from LL patients compared with healthy subjects. Flow cytometry showed no signifi - cant difference in IFN-γR1 expression between LL patients and healthy subjects. Immunoblotting detected only the mature glycosylated form of the 61-67 kDa IFN-γR2 protein in healthy subjects. In contrast, cells from LL patients showed three different expression patterns: (1) the immature deglycosylated form of the 34.8 kDa IFN- γR2 protein, (2) the mature glycosylated 61-67 kDa form, and (3) both forms. Our data indicate the existence of abnormalities in the intracellular processing and protein expression of the IFN-γR in response to specifi c stimuli such as IFN-γ and M. leprae membrane proteins in adherent cells of LL patients. © 2009 Mary Ann Liebert, Inc.
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