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Title: Treatment with anti-transforming growth factor ? antibodies influences an altered pattern of cytokines gene expression in injured rat liver
Author: Delgado-Rizo, V.
Salazar, A.
Panduro-Cerda, Arturo
Armendariz-Borunda, J.
Issue Date: 1998
Abstract: The role of transforming growth factor ?1 (TGF-?1) in mediating hepatic inflammation and regeneration after acute liver injury is beginning to be elucidated, yet its in vivo effect on the gene expression of the major pro-inflammatory and antiinflammatory cytokines produced during that process is unknown. Our previous experiments demonstrated that anti-TGF-?-treated animals presented profound histological changes as compared with control animals. Therefore, our hypothesis was that by blocking in vivo TGF-?1 action, with polyclonal anti-TGF-? antibodies, we could monitor by RT-PCR significative alterations on the gene expression of IL-1?, IL-6, TGF-?, TNF-?, IL-4 and IL-10 in liver-regenerated rats after administration of a single CCl4 dosing. Accordingly, we here report a completely different pattern of cytokines gene expression amidst those groups of rats. Pro- inflammatory cytokines gene expression in control animals showed a clear-cut pattern peaking at 1-2 days postinjury and declining thereafter. Interestingly, IL-6 was present in the control animals only between 12 and 24 h after CCl4 dosing. In the experimental animals, TGF-?1 was mainly increased at 4 and 6 days, while IL-6 mRNA was completely absent. IL-l? mRNA expression was also altered in the experimental rats, albeit TNF-? was nearly unaffected. IL-4 was fully absent in control rats, but remarkably expressed in experimental animals throughout the study. IL-10 was also more expressed in experimental animals.
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