Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.12104/44639
Title: Somatic embryogenesis in Agave tequilana Weber cultivar azul
Author: Portillo, L.
Santacruz-Ruvalcaba, F.
Gutierrez-Mora, A.
Rodriguez-Garay, B.
Issue Date: 2007
Abstract: Somatic embryogenesis was achieved from leaves of Agave tequilana Weber cultivar azul utilizing MS medium supplemented with L2 vitamins and the addition of cytokinins: 6-benzylaminopurine (BA), 1-phenyl-3(1,2,3-thiadiazol-5-yl)urea (TDZ), 6-(?-?-dimethylamino)purine (2ip) and 6-furfurylaminopurine (KIN), combined with the auxin 2,4-dichlorophenoxyacetic acid (2,4-D). Differences among the six genotypes studied with regard to their embryogenic response in culture were found. Embryos produced by genotype S3 under a hormone regime of high cytokinin (44.4 to 66.6 ?M BA) compared to auxin (4.5 ?M 2,4-D) contained chlorophyll, whereas those produced when auxin was high compared to cytokinin (9.0 and 13.6 ?M 2,4-D and 1.3 and 4.0 ?M BA, respectively) were whitish and morphologically similar to their zygotic counterparts. Somatic embryos matured and germinated after transferring the embryogenic calli to maturation and germination medium without growth regulators and enriched with organic nitrogen. Microscopic observations demonstrated a unicellular origin for production of indirect somatic embryos. � 2007 The Society for In Vitro Biology.
URI: http://www.scopus.com/inward/record.url?eid=2-s2.0-37249005305&partnerID=40&md5=e0be63a023f9b5c744936bec60a1f799
http://dx.doi.org/10.1007/s11627-007-9046-5
http://hdl.handle.net/20.500.12104/44639
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