Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.12104/43938
Title: Protease-activated receptor-2 (PAR-2) in cervical cancer proliferation
Author: Sanchez-Hernandez, P.E.
Ramirez-Duenas, M.G.
Albarran-Somoza, B.
Garcia-Iglesias, T.
del Toro-Arreola, A.
Franco-Topete, R.
Daneri-Navarro, Adrián
Issue Date: 2008
Abstract: Objective.: Protease-activated receptor-2 (PAR-2) is a G-protein-coupled receptor that is cleaved and activated by trypsin and tryptase. There is evidence that PAR-2 contributes to tumor progression in stomach, colon, pancreas, prostate and breast cancer patients. However, the role of PAR-2 in cervical cancer is still unknown. The aim of this work was to study the PAR-2 expression in cervical cancer tissues and the effect of PAR-2 activation on cervical cancer proliferation. Methods.: Immunohistochemistry was used to analyze PAR-2 expression in fixed paraffin-embedded tumor tissue from 16 patients with invasive cervical cancer. HPV types were identified by PCR. PAR-2 expression in UISO-SQC-1, HeLa, SiHa, CasKi and C-33 A cervical cancer cell lines was evaluated by flow cytometry. Trypsin was detected by Western blot. Tumor proliferation in response to trypsin or agonist peptide was evaluated by the MTT method. Results.: A strong correlation between trypsin and PAR-2 expression in five cervical cancer cell lines, in association with proliferative growth in the presence of trypsin or agonist peptide, was found. All tumors from cervical cancer patients expressed PAR-2 (immunoreactive score was higher in poorly differentiated tumors). Conclusions.: Results suggest that trypsin and PAR-2 are involved in cervical cancer cell proliferation. © 2007 Elsevier Inc. All rights reserved.
URI: http://www.scopus.com/inward/record.url?eid=2-s2.0-37449024269&partnerID=40&md5=0fea950cd8466c50ed4d3b3f9c46e71c
http://ovidsp.ovid.com/ovidweb.cgi?T=JS&CSC=Y&NEWS=N&PAGE=fulltext&D=med5&AN=17936340
http://hdl.handle.net/20.500.12104/43938
Appears in Collections:Producción científica UdeG

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