Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.12104/42197
Title: Optimization of DNA isolation and PCR protocol for analysis and evaluation of genetic diversity of the medicinal plant, Anemopsis californica using RAPD
Author: Silva, J.M.
Ortega-Gudino, P.
Rabelero, M.
Sanchez-Diaz, J.C.
Guerrero-Ramirez, L.G.
Puig, J.E.
Issue Date: 2013
Abstract: The synthesis of polyacrylamide nanoparticles by semi-continuous inverse heterophase polymerization as a function of feeding rate of monomer aqueous solution is reported here. In this process, a concentrated acrylamide aqueous solution is dosed semi-continuously at various rates over an AOT-toluene solution containing the initiator. Our results indicate that particle size and the viscosimetric molar masses diminish as the dosing rate is slowed down and that smaller particles, as well as lower molar masses, are obtained compared to those produced by batch and semi-continuous microemulsion polymerizations, employing the same concentration of surfactant. Moreover, higher polymer/surfactant ratios are higher compared to those obtained in batch microemulsion polymerization and similar or slightly higher than that in semi-continuous microemulsion polymerization. Copyright " Taylor & Francis Group, LLC.",,,,,,"10.1080/10601325.2013.784548",,,"http://hdl.handle.net/20.500.12104/42197","http://www.scopus.com/inward/record.url?eid=2-s2.0-84877138552&partnerID=40&md5=e14d49c8fc59b3cbc5a6473a6980f0e7",,,,,,"6",,"Journal of Macromolecular Science, Part A: Pure and Applied Chemistry",,"596
601",,"50",,"Scopus
WOS",,,,,,"inverse heterophase polymerization; latexes nanoparticles; Polyacrylamide",,,,,,"Increasing solid content in latexes of polyacrylamide nanoparticles made by semi-continuous inverse heterophase polymerization",,"Article" "44032","123456789/35008","Larios, F.E., Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Jalisco, Mexico; Robles, R., Instituto para el Fortalecimiento de Capacidades en Salud: FOCUS Salud México S.C., Convento San Juan de los Lagos, 97, Col. Jardines de Santa Mónica, Tlalnepantla, Estado de México. 54050, Mexico; Jiménez, L.M., Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Jalisco, Mexico; Suárez, Á.E., Hospital General No. 45, Instituto Mexicano del Seguro Social, Mexico; Del Toro, A., Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Jalisco, Mexico; Daneri, A., Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Jalisco, Mexico
Daneri-Navarro, Adrián., Universidad de Guadalajara. Centro Universitario de Ciencias de la Salud",,"Larios, F.E.
Robles, R.
Jimenez, L.M.
Suarez, A.E.
Del Toro, A.
Daneri-Navarro, Adrián.",,"2009",,"The aim of the present study was to develop and to evaluate a guide to communicate the presence and treatment of Papanicolaou alterations that suggest dysplasia or cervical cancer. Method: A sample of 97 women with abnormal smear test referred to diagnosis and treatment at a third level hospital were interviewed pre and post an informative session systematized in the proposed guide, to compare the degree of information about their condition and treatment and their perception of emotional support from health professionals. Results: The communication guide were developed according evidence based treatment model. We documented a pre-post clinical and statistical difference in the level of information about their condition and treatment (3.97 ± 3.15 vs. 8.15 ± 2.65; t= -7.27, df= 53, p?.0001), and a tendency of increment in the percentage of patients that perceived emotional support from health professionals (62.3% vs. 100%; chi square=31.18, df=1, p=.07). Conclusions: The guide presented here constitutes a useful material to improve communication of these conditions and its treatment among Hispanic women with low level of education.",,,,,,,,,,"http://www.scopus.com/inward/record.url?eid=2-s2.0-67649908902&partnerID=40&md5=5a12a547330db261825e55c9b9e6f520
http://hdl.handle.net/20.500.12104/42253",,,,,,"1",,"Psicooncologia",,"155
166",,"6",,"Scopus",,,,,,"Cervical cancer; Communication; Emotional support; Heath education; Papanicolaou; Psychoeducation",,,,"Information and emotional support for women with abnormal pap test results [Información y soporte emocional a las mujeres con resultado anormal en Papanicolaou: Una guía de comunicación]",,,,"Article", "43993","123456789/35008",,"Klimov, A.B., Departamento de Fisica, Universidad de Guadalajara, Revolución 1500, 44420 Guadalajara, Jalisco, Mexico; De Guise, H., Department of Physics, Lakehead University, Thunder Bay, Ont. P7B 5E1, Canada; Sánchez-Soto, L.L., Departamento de óptica, Facultad de Física, Universidad Complutense, 28040 Madrid, Spain
Klimov, Andrei B., Universidad de Guadalajara. Centro Universitario de Ciencias Exactas e Ingenierías",,"Klimov, Andrei B.
De Guise, H.
Sanchez-Soto, L.L.",,"2003",,"A study was performed to demonstrate that the ? and V configurations cannot be taken as equivalent if treating the photon field as a quantized field. It was also possible to see why, physically, these configurations are different: in a V configuration, vacuum fluctuations can create a photon, the absorption of which acts as a trigger for the transfer of excitation between levels |2> and |3>.",,,,,,,,,"http://hdl.handle.net/20.500.12104/42214","http://www.scopus.com/inward/record.url?eid=2-s2.0-1142303889&partnerID=40&md5=a80f45d29651ce797562eaea025ca488",,,,,,"6",,"Physical Review A - Atomic, Molecular, and Optical Physics",,"065801/1
065801/4",,"68",,"Scopus
WOS",,,,,,,,,,,,"Inequivalent classes of closed three-level systems",,"Article" "43992","123456789/35008","Díaz, J.A.R., Departamento de Estudios en Educación, Centro Universitario de Ciencias Sociales y Humanidades, Universidad de Guadalajara, Guanajuato núm. 1045Guadalajara, Jalisco, Mexico",,"Diaz, J.A.R.",,"2015",,"[No abstract available]",,,,,,,,,,"http://www.scopus.com/inward/record.url?eid=2-s2.0-84919423023&partnerID=40&md5=319226d3e04b310b506426ababa935a6
http://hdl.handle.net/20.500.12104/42213",,,,,,"64",,"Revista Mexicana de Investigacion Educativa",,"11
16",,"20",,"Scopus",,,,,,,,,,"Inequity in higher education in Latin America [Las inequidades en la educación superior en Iberoamérica]",,,,"Editorial", "45179","123456789/35008",,"Lizette Del-Toro-Sánchez, C., Department of Medical Sciences and Life, University Center of the Cienega, University of Guadalajara, 1115 University Av, 47820 Ocotlán, Jalisco, Mexico; Villaseñor-Alvarado, S., Department of Medical Sciences and Life, University Center of the Cienega, University of Guadalajara, 1115 University Av, 47820 Ocotlán, Jalisco, Mexico; Zurita-Martínez, F., Department of Medical Sciences and Life, University Center of the Cienega, University of Guadalajara, 1115 University Av, 47820 Ocotlán, Jalisco, Mexico; Castellanos-Hernández, O.A., Department of Medical Sciences and Life, University Center of the Cienega, University of Guadalajara, 1115 University Av, 47820 Ocotlán, Jalisco, Mexico; Rodríguez-Sahagún, A., Department of Medical Sciences and Life, University Center of the Cienega, University of Guadalajara, 1115 University Av, 47820 Ocotlán, Jalisco, Mexico; Isabel Torres-Morán, M., University Center of Biological and Agricultural Sciences, University of Guadalajara, 15.5 km of Highway Guadalajara-Nogales, 45110 Zapopan, Jalisco, Mexico; Rojas-Bravo, D., Department of Medical Sciences and Life, University Center of the Cienega, University of Guadalajara, 1115 University Av, 47820 Ocotlán, Jalisco, Mexico; Gutiérrez-Lomelí, M., Department of Medical Sciences and Life, University Center of the Cienega, University of Guadalajara, 1115 University Av, 47820 Ocotlán, Jalisco, Mexico",,"Lizette Del-Toro-Sanchez, C.
Villasenor-Alvarado, S.
Zurita-Martinez, F.
Castellanos-Hernandez, O.A.
Rodriguez-Sahagun, A.
Isabel Torres-Moran, M.
Rojas-Bravo, D.
Gutierrez-Lomeli, M.",,"2013",,"Anemopsis californica is a perennial herbaceous plant that has been utilized as a medicinal plant for the treatment of various diseases. The present work was carried out with the objective of optimizing a method of extraction of the genomic DNA of A. californica and a PCR protocol and later to evaluate the existing genetic diversity among the genotypes deriving from different origins. For DNA extraction, we tested four procedures: with the CTA B-2 protocol, we obtained the highest yield (61.5 2.2 ?g DNA/g of leaf tissues) and the best quality (A260/280 1.83 0.022). To estimate genetic variability, we utilized the randomly amplified polymorphism DNA (RAPD) technique, employing 20 oligonucleotides, of which only 18 generated reproducible banding patterns, producing 123 polymorphic bands generated, thus obtaining a polymorphism rate of 93.93% among the genotypes analyzed. The Jaccard similarity coefficient generated a variation ranging from 0.325-0.921, indicating a high level of genetic variation among the studied genotypes. An Unweighted pair-group method with arithmetic mean (UPGMA) group analysis indicated six distinct groups. The present optimized method for DNA isolation and RAPD protocol may serve as an efficient tool for further molecular studies. " 2013 Akademiai Kiadó, Budapest.
URI: http://hdl.handle.net/20.500.12104/43400
http://www.scopus.com/inward/record.url?eid=2-s2.0-84878780792&partnerID=40&md5=a22003fb4a4b740ff6e2ce9744ff6db9
http://ovidsp.ovid.com/ovidweb.cgi?T=JS&CSC=Y&NEWS=N&PAGE=fulltext&D=medl&AN=23739887
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