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Title: Identification of immunodominant antigens of Mycobacterium bovis by expression library immunization
Author: Beltran, P.K.
Gutierrez-Ortega, A.
Puebla-Perez, A.M.
Gutierrez-Pabello, J.A.
Flores-Valdez, M.A.
Hernandez-Gutierrez, R.
Martinez-Velazquez, M.
Alvarez, A.H.
Issue Date: 2011
Abstract: This study combines two methodologies - vector expression of a genomic library and proteomics - to identify immunogenic proteins of Mycobacterium bovis. Immunization of BALB/c mice with a plasmid DNA pool from the library, containing approximately 8000 clones, induced a humoral response that facilitated the detection of 12 antigenic proteins by Western blotting. Two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and mass spectrometry identified four proteins (Cpn60-1, HSP70, EF-Tu, and AdoHcyase). Such genomic immunization offers the possibility of in vivo screening of potential candidate M. bovis antigens. � 2010 Elsevier Ltd.
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