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Title: High yield and high quality DNA from vegetative and sexual tissues of Mexican white pine (Pinus ayacahuite)
Author: Contreras, A.M.
Ochoa-Jimenez, R.J.
Celis, A.
Mendez, C.
Olivares, L.
Rebolledo, C.E.
Hernandez-Lugo, I.
Aguirre-Zavala, A.I.
Jimenez-Mendez, R.
Chung, R.T.
Issue Date: 2010
Abstract: Background: The screening and diagnosis of hepatitis C virus (HCV) infection is initiated by testing for antibody to HCV (anti-HCV). A positive anti-HCV test in blood donors represents ongoing infection in only a variable proportion of individuals. Because a high anti-HCV level has been associated with viremia, a study was conducted to determine whether a high antibody level is an accurate serologic marker for viremia in asymptomatic anti-HCV-positive persons. Study design and methods: In a diagnostic test study, we included 856 anti-HCV-positive blood donors in a blood bank at Guadalajara, Jalisco, Mexico, between 2002 and 2007. A third-generation amplified chemiluminescence assay (ChLIA HCV) was used to detect anti-HCV. A positive result of the qualitative nucleic acid test (HCV RNA) was considered the gold standard for viremia. RESULTS: By receiver operating characteristic analysis, the signal-to-cutoff (S/CO) ratio of 20 or more was chosen as optimal to identify viremia and so was defined as high anti-HCV level. There was a significant difference in the proportion of viremia between subjects with high antibody level and those with lower levels (93.7% vs. 1.8%, respectively; p < 0.001). A high antibody level showed a sensitivity for viremia of 96.6% (95% confidence interval [CI], 93.8%-98.1%), a specificity of 96.6% (95% CI, 94.8%-97.8%), and a likelihood ratio of 28.6 (95% CI, 18.4%-44.6%). CONCLUSION: A high antibody level (S/CO ratio ?20 by ChLIA HCV) clearly divides the viremic from the nonviremic blood donors and functions as an accurate serologic marker to guide the use of routine HCV RNA testing to confirm hepatitis C infection. " 2010 American Association of Blood Banks.",,,,,,"10.1111/j.1537-2995.2009.02571.x",,,"","",,,,,,"6",,"Transfusion",,"1335
WOS",,,,,,,,,,,,"High antibody level: An accurate serologic marker of viremia in asymptomatic people with hepatitis C infection",,"Article" "43696","123456789/35008",,"Palomera-Avalos, V., Departamento de Biología Celulary Molecular, Universidad de Guadalajara, 45110, Zapopan, Jalisco, Mexico; Castro-Félix, P., Departamento de Biología Celulary Molecular, Universidad de Guadalajara, 45110, Zapopan, Jalisco, Mexico; Villalobos-Arámbula, A.R., Departamento de Biología Celulary Molecular, Universidad de Guadalajara, 45110, Zapopan, Jalisco, Mexico",,"Palomera-Avalos, V.
Castro-Felix, P.
Villalobos-Arambula, A.R.",,"2008",,"Pines are considered to be difficult for DNA extraction. However, from one species to the other there is variation in phenolic profiles and seed size that might affect final DNA yields and quality. Two DNA extraction protocols (CTAB and SDS based) were compared for their ability to produce DNA on leaves, gametophyte and embryo from Pinus ayacahuite, a pine species with a large seed (8 - 18 mm). The DNA obtained from both procedures was quantified and tested by PCR. The CTAB protocol provided higher DNA yields from vegetative tissue and embryo than the SDS method. Embryos (2n) and gametophytes (n) proved to be very good sources of DNA and the DNA isolated was suitable for PCR-RAPD and SSR markers. This paper reports the results and describes the modified CTAB protocol. " 2008 Academic Journals.
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