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Title: Nuclear genome size and cytotype analysis in Agave cupreata Trel. & Berger (Agavaceae)
Author: Armendariz-Borunda, J.
Rincon, A.R.
Munoz-Valle, J.F.
Bueno-Topete, M.
Oregon-Romero, E.
Islas-Carbajal, M.C.
Medina-Preciado, D.
Gonzalez-García, I.
Bautista, A.
García-Rocha, S.
Godoy, J.
Mercado, M.V.-D.
Troyo-SanRoman, R.
Arellano-Olivera, I.
Lucano, S.
Alvarez-Rodriguez, A.
Salazar, A.
Issue Date: 2008
Abstract: Background/Aim: The aim of this work was to establish a potential correlation between specific polymorphisms and presence of hepatic fibrosis in Mexican patients with established liver fibrosis (ELF). Second, necroinflammatory index improvement was correlated with Pirfenidone (PFD) treatment response and the same polymorphisms. Methods: We analyzed TGF-? polymorphisms in codon 25, a single basepair guanine insertion-deletion polymorphism (4G/5G) for PAI-1 and angiotensin AT-6 single nucleotide polymorphism located in -6 promoter region. Twenty patients infected with either hepatitis C virus (HCV) (n = 13) or affected by alcohol consumption (n = 7) were included. Thirty subjects with no hepatic damage were included in control group. Blood samples for genomic DNA were obtained and plasminogen activator inhibitor-1 polymorphisms were done by polymerase chain reaction-artificial introduction of a restriction site, TGF-? by polymerase chain reaction-amplification refractory mutation system and AT by polymerase chain reaction-restriction fragment length polymorphisms. Liver biopsies were obtained at baseline and after 12 months of PFD treatment. Results: Established liver fibrosis patients had the homo-zygote G/G TGF-? genotype, which has been associated with increased development of fibrosis. None of our patients had the G/C genotype. All pure HCV and pure alcohol abuse subjects carried G/G TGF-? genotype (100% vs 37% control) (P = 0.0006). The odds of having ? G/G genotype was 19.5 for HCV patients and 10.83 for alcohol consumption patients as compared with healthy subjects (P < 0.001). Established liver fibrosis patients had an improvement in necroinflammatory index after PFD treatment when correlated with plasminogen activator inhibitor-1 and angiotensinogen-6 genotypes. Conclusion: Our data suggested that a combination of inherited polymorphisms increased the risk of advanced fibrosis in ELF patients. Pure HCV and pure alcohol consumption patients which were homozygous G/G carriers had 19.5- and 10.8-fold higher risk to develop advanced fibrosis respectively. " 2008 by The American Federation for Medical Research.",,,,,,,,,"","",,,,,,"7",,"Journal of Investigative Medicine",,"944
WOS",,,,"Index Medicus;Adult;Aged;Angiotensinogen/ge [Genetics];Anti-Inflammatory Agents, Non-Steroidal/tu [Therapeutic Use];Base Sequence;DNA Primers/ge [Genetics];Female;Hepatitis C/dt [Drug Therapy];Hepatitis C/ge [Genetics];Humans;Liver Cirrhosis/dt [Drug Therapy];Liver Cirrhosis/ge [Genetics];Liver Cirrhosis, Alcoholic/dt [Drug Therapy];Liver Cirrhosis, Alcoholic/ge [Genetics];Male;Mexico;Middle Aged;Plasminogen Activator Inhibitor 1/ge [Genetics];Polymorphism, Genetic;Pyridones/tu [Therapeutic Use];Transforming Growth Factor beta/ge [Genetics]",,"AT; Cirrhosis; PAI-1; Pirfenidone; Polymorphisms; TGF-?",,,,,,"Fibrogenic polymorphisms (TGF-?, PAI-1, AT) in Mexican patients with established liver fibrosis. Potential correlation with pirfenidone treatment",,"Article" "45031","123456789/35008",,"Palomino, G., Instituto de Biología, Jardín Botúnico, Universidad Nacional Autónoma de México, México D. F. 04510, Mexico; Martínez, J., Instituto de Biología, Jardín Botúnico, Universidad Nacional Autónoma de México, México D. F. 04510, Mexico; Cepeda-Cornejo, V., Instituto de Biología, Jardín Botúnico, Universidad Nacional Autónoma de México, México D. F. 04510, Mexico, Instituto de Ecología, Universidad Nacional Autónoma de México, México D. F., Mexico; Pimienta-Barrios, E., Departamento de Ecología, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara 45110, Zapopan, Jalisco, Mexico",,"Palomino, G.
Martinez, J.
Cepeda-Cornejo, V.
Pimienta-Barrios, E.",,"2012",,"This study is a cytogenetic characterization by karyotyping and a determination of the DNA content by flow cytometry of wild populations of Agave cupreata from the Guerrero State, Mexico. Three of the studied populations were diploids (2n = 2x =60) and one population had tetraploid (2n = 4x = 120), pentaploid (2n = 5x = 150) and hexaploid (2n = 6x = 180) plants. Diploid populations had three different structural cytotypes. One population showed polyploid cytotypes. A. cupre-ata showed a bimodal karyotype of 10 large + 50 small chromosomes in diploids; 20 large + 100 small chromosomes in tetraploids and 25 large + 125 small chromosomes in pentaploids. In diploids, they had secondary constriction in one pair of the large chromosomes and, in the fourth or fifth large homologous chromosome groups, in polyploid plants. The arm ratio, the proportion of different types of large and small chromosomes, the mean of genome length and the asymmetry index of karyotypes clearly varied among diploid and polyploid cytotypes. The pattern of variation among Agave cupreata populations is probably due to rearrangements in the large and small chromosomes of the complement. The diploid populations displayed a 0.63% variation in 2C DNA content. The mean 2C DNA content was 7.88 pg; 1Cx value = 3.94 pg in diploids of Agave cupreata. Tetraploids had 2C = 16.54 pg DNA, i.e. approximately four times the 1Cx value; 2C DNA amounts of pentaploid and hexaploid were equal to 20.42 and 23.92 pg DNA, respectively. These values are also multiples of 1Cx value, indicating a relationship between the ploidy level and the 2C DNA content. The results shown are basic and useful information to develop biotechnology and breeding approaches for Agave cupreata. " 2012 Dipartimento di Biologia Evoluzionistica, University di Firenze.
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