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|Title:||Effect of the use of a neutralizing step after antimicrobial application on microbial counts during challenge studies for orange disinfection|
|Author:||Montano Loza, A.|
Rodriguez Lomeli, X.
García Correa, J.E.
Davalos Cobian, C.
Cervantes Guevara, G.
Medrano Munoz, F.
Fuentes Orozco, C.
Gonzalez Ojeda, A.
|Abstract:||Background: hyperamylasemia and acute pancreatitis represent the most frequent major complication after endoscopic retrograde cholangiopancreatography (ERCP), developing in 1-30% of cases. Objective: to determine the incidence of hyperamylasemia and acute pancreatitis after ERCP, and to assess the utility of rectal indomethacin to prevent these events. Material and methods: a randomized clinical trial. During a 12-month period 150 patients were included. They were divided up into a study group (n = 75), where 100 mg of rectal indomethacin were administered 2 hours prior to the procedure, and a control group (n = 75), which received rectal glycerin. Two hours after ERCP serum amylase levels were measured and classified as follows: 0 ? 150 IU/L, 1 = 151-599 IU/L, 2 ? 600 IU/L. Clinical pancreatitis episodes were quantified and classified according to Ranson's criteria. Results: gender distribution: 100 women and 50 men. Mean age: 55.37 18.0 for the study group, and 51.1 17.0 for the control group. A diagnosis of benign pathology was present in 56 (74.7%) cases in the study group, and 59 (78.7%) controls. After ERCP 13 (17.3%) patients in the study group and 28 (37.3%) in the control group developed hyperamylasemia (p ? 0.05). Hyperamylasemia > 600 IU/L was found in 3 patients in the study group, and in 10 in the control group (p = 0.001). Mild pancreatitis was detected in 4 (5.3%) patients in the study group, and in 12 (16%) patients in the control group (p = 0.034). There were no deaths or adverse drug reactions. Conclusions: rectal indomethacin before ERCP decreases the risk of hyperamylasemia and pancreatitis. Indomethacine is a feasible, low-cost drug with minimal or nil side effects. Copyright " 2007 Arán Ediciones, s. l.",,,,,,,,,"http://hdl.handle.net/20.500.12104/41035","http://www.scopus.com/inward/record.url?eid=2-s2.0-35848938503&partnerID=40&md5=6587a230ebd1d578f02524aa2b9d80d0",,,,,,"6",,"Revista Espanola de Enfermedades Digestivas",,"330|
336",,"99",,"Scopus",,,,,,"Acute pancreatitis; Endoscopic retrograde cholangiopancreatography; Hyperamylasemia; Indomethacin",,,,,,"Effect of the rectal administration of indomethacin on amylase serum levels after endoscopic retrograde cholangiopancreatography, and its impact on the development of secondary pancreatitis episodes [Efecto de la administración de indometacina rectal sobre los niveles síricos de amilasa posteriores a colangiopancreatografía retrógrada endoscópica y su impacto en la aparición de episodios de pancreatitis secundaria]",,"Article" "42816","123456789/35008",,"Montañez Valdez, O.D., Departamento de Desarrollo Regional, CUCSUR, Universidad de Guadalajara, Av. Prolongacion Colon S/N, C.P. 49000 CD. Guzmán, Mexico; García Flores, E.O., Centro de Investigaciones Pecuarias, Dpto. de Producción agrícola CUCSUR, Universidad de Guadalajara, Autlán, Jalisco, Mexico; Peralta Ortiz, J.G., Colegio de Postgraduados, Programa de Ganadería, Montecillo, Texcoco, Mexico; García Martínez, J.E., Departamento de Nutrición Animal y Alimentos, UAAAN, Buenavista, Saltillo, Coahuila, Mexico; Rojo Rubio, R., Centro Universitario Temascaltepec, Universidad Autónoma del Estado de México, Temascaltepec, Mexico; Avellaneda-Cevallos, J.H., Unidad de Investigación, Facultad de Ciencias Pecuarias, Universidad Técnica Estatal de Quevedo, Quevedo, Los Ríos, Ecuador",,"Montanez Valdez, O.D.
García Flores, E.O.
Peralta Ortiz, J.G.
García Martinez, J.E.
Rojo Rubio, R.
Avellaneda-Cevallos, J.H.",,"2006",,"The objective of this study was to evaluate the effect of B vitamins on in vitro digestibility of dry matter (IVDMD) and organic matter (IVOMD) of diets with different source of carbohydrates (sorghum and barley grain), with five concentrations of B vitamins (BV; 0, 2, 4, 6 and 8 g kg-1 DM). There were differences (P < 0.01) for the principal effects (source of carbohydrates x concentration of BV), also for the interaction of them in both cases (IVDMD and IVOMD). At 8 g with sorghum increases the IVDMD and IVOMD, but did not change when the source of carbohydrates was the barley. The addition of B vitamins improves the IVDMD and IVOMD, also the nature and quality of the diet effect the microbial B vitamin production.",,,,,,,,,"http://hdl.handle.net/20.500.12104/41037","http://www.scopus.com/inward/record.url?eid=2-s2.0-33750731624&partnerID=40&md5=ce47c81de705c87874ee1b5171433e9e",,,,,,"5",,"Revista Cientifica de la Facultad de Ciencias Veterinarias de la Universidad del Zulia",,"531
WOS",,,,,,"B vitamin; Digestibility; In vitro",,,,,,"Effect of the supplementation of B vitamins on in vitro digestibility of diets with different source of carbohydrates [Efecto de la adición de vitaminas B en la digestibilidad in vitro de dietas con diferente fuente de carbohidratos]",,"Article" "42817","123456789/35008",,,,"Naranjo González, Mario Alberto",,"2002",,,,,,,,,,"1405-9282","http://hdl.handle.net/20.500.12104/41038",,,"Español",,,,"10",,"Revista Mexicana de Agronegocios",,"374-386",,"6",,"CLASE",,,,,,,,"Administración de instituciones",,,"El control estadístico de procesos","El control estadístico de procesos: una herramienta para mejorar la organización y la calidad de la empresa",,"journalArticle" "42818","123456789/35008",,"Martínez-Gonzáles, N.E., Departamento de Farmacobiología, Universidad de Guadalajara, Guadalajara, Jalisco 44430, Mexico; Martínez-Cárdenas, C., Departamento de Farmacobiología, Universidad de Guadalajara, Guadalajara, Jalisco 44430, Mexico; Martínez-Chávez, L., Departamento de Farmacobiología, Universidad de Guadalajara, Guadalajara, Jalisco 44430, Mexico; Ramos-Pérez, N.E., Departamento de Farmacobiología, Universidad de Guadalajara, Guadalajara, Jalisco 44430, Mexico; Taylor, T.M., Department of Animal Science, Texas A and M University, College Station, TX 77843-2471, United States; Ulloa-Franco, C.C., Departamento de Farmacobiología, Universidad de Guadalajara, Guadalajara, Jalisco 44430, Mexico; Castillo, A., Department of Animal Science, Texas A and M University, College Station, TX 77843-2471, United States",,"Martinez-Gonzales, N.E.
Castillo, A.",,"2013",,"The effects of using a neutralizer after applying antimicrobial treatments and the effect of time lapse between treatment application and subsequent recovery and enumeration of Escherichia coli O157:H7 and Salmonella were investigated in Valencia oranges. Inoculated oranges surfaces were washed with distilled water for 15 s and then sprayed with a solution containing 200 mg/liter sodium hypochlorite (pH 6.5) for 15 s; they were then dipped in L-lactic acid (2.0% at 55°C) for 1 min or in distilled water at 80°C for 1 min. Posttreatment, oranges were divided into two groups. In the first group, oranges were dipped in neutralization treatment: 270 ml of buffered peptone water for 2 min for lactic acid-treated oranges, 270 ml of Dey-Engley broth for 2 min for chlorine-treated oranges, or 3.7 liters of tap water (25°C) for 10 s for hot water-treated oranges. The second group of treated oranges was not subjected to any neutralizer. All oranges then were kept at room temperature (average 26.2°C) and sampled at 0, 7.5, and 15 min for enumeration of surviving Salmonella and E. coli O157:H7. The orange surface (30 cm2) was excised for pathogen enumeration. The presence of free chlorine and changes in pH and temperature on the orange surface were determined in uninoculated, treated oranges. Free chlorine was detected on oranges after treatment; the change in temperature of orange surfaces was greater during treatment with hot water than with lactic acid. Nevertheless, pathogen enumeration did not show any impact of neutralizer use on the residual activity of antimicrobials or any impact of the time elapsed between antimicrobial treatment and recovery of bacterial pathogens from inoculated oranges (P ? 0.05). The results of this study indicate that the lack of a neutralizing step before enumeration of pathogens is not likely to affect the accuracy of results during challenge studies to test pathogen reduction strategies on oranges. Copyright ", International Association for Food Protection.
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