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|dc.description.abstract||Background: Severe acute pancreatitis (SAP) is associated with high morbidity and mortality. Objective: To evaluate whether necrosectomy, alone or combined with vacuum-assisted closure (VAC), has any additional beneficial effects on mitochondrial function and/or oxidative stress markers in SAP. Methods: Patients with SAP, APACHE II score > 8, and inadequate response to management in an intensive care unit were included in a prospective observational study. Sixteen underwent necrosectomy and 24 underwent necrosectomy plus VAC every 48 h. Patients were then categorized as survivors or deceased. Submitochondrial membrane fluidity of platelets and F<inf>0</inf>F<inf>1</inf>-ATPase hydrolysis were measured to represent mitochondrial function. Oxidative/nitrosative stress was measured using lipoperoxides (LPOs), nitric oxide (NO), erythrocyte membrane fluidity, and total antioxidant capacity (TAC). Results: Membrane fluidity in submitochondrial particles of platelets remained significantly increased throughout the study, and then eventually rised in deceased patients managed with necrosectomy + VAC vs. survivors (p < 0.041). Hydrolysis was significantly increased from baseline to endpoint in all patients, predominating in those who died after management with necrosectomy (p < 0.03). LPO increased in all patients, and necrosectomy was more efficient for the eventual decrease in survivors (p < 0.039). NO was found to be increased for the baseline-endpoint result among both survivors and deceased patients with both management options. Erythrocyte membrane fluidity was increased in survivors managed with necrosectomy + VAC, and eventually returned to normal (p < 0.045). TAC was found to be consumed in all patients for the duration of the study. Conclusions: Mitochondrial dysfunction and oxidative?nitrosative stress with significant systemic antioxidant consumption were found. Necrosectomy was more efficient and better cleared LPOs. Necrosectomy + VAC improved erythrocyte membrane fluidity and increased survival. � 2014 Ar�n Ediciones, S. L.|
|dc.title||Effect of necrosectomy and vacuum-assisted closure (VAC) on mitochondrial function and oxidative stress markers in severe acute pancreatitis|
|dc.relation.ispartofjournal||Revista Espanola de Enfermedades Digestivas|
|dc.subject.keyword||Acute pancreatitis; Mitochondrial dysfunction; Oxidative stress; Severe acute pancreatitis|
|dc.contributor.affiliation||Miranda-D�az, A.G., Department of Physiology, Centro Universitario de Ciencias de la Salud, Universidad de GuadalajaraGuadalajara, Jalisco, Mexico; Hermosillo-Sandoval, J.M., Department of General Surgery, Centro M�dico Nacional de Occidente, Instituto Mexicano del Seguro SocialGuadalajara, Jalisco, Mexico; Guti�rrez-Mart�nez, C.A., Intensive Care Unit. Hospital de Especialidades, Centro M�dico Nacional de Occidente, Instituto Mexicano del Seguro SocialGuadalajara, Jalisco, Mexico; Rodr�guez-Carrizalez, A.D., Department of Physiology, Centro Universitario de Ciencias de la Salud, Universidad de GuadalajaraGuadalajara, Jalisco, Mexico; Rom�n-Pintos, L.M., Department of Physiology, Centro Universitario de Ciencias de la Salud, Universidad de GuadalajaraGuadalajara, Jalisco, Mexico; Cardona-Mu�oz, E.G., Department of Physiology, Centro Universitario de Ciencias de la Salud, Universidad de GuadalajaraGuadalajara, Jalisco, Mexico; Pacheco-Mois�s, F.P., Department of Chemistry, Universidad de GuadalajaraGuadalajara, Jalisco, Mexico; Arias-Carvajal, �., Department of Physiology, Centro Universitario de Ciencias de la Salud, Universidad de GuadalajaraGuadalajara, Jalisco, Mexico|
|dc.subject.keywordspa||Pancreatitis aguda; Disfunci�n mitocondrial; Estr�s oxidativo; Pancreatitis aguda severa|
|dc.subject.heading||Gastroenterology & Hepatology|
|Appears in Collections:||Producción científica UdeG|
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