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Title: Characterization of DNA/Buffer/H2O system through Electrochemical Impedance Spectroscopy (EIS)
Author: Bravo-Anaya, L.M.
Larios-Duran, E.R.
Casillas, N.
Carvajal, F.
Fernandez Escamilla, V.V.
Macias-Balleza, E.R.
Soltero, J.F.A.
Issue Date: 2013
Abstract: In this work we present a study of transitional behavior of calf-thymus DNA molecules in solution in a concentration range from 0.01 to 2.5 mg mL -1 at different temperatures (10, 20, 30 and 40 �C) and a pH of 7.3 using Electrochemical Impedance Spectroscopy (EIS). The results are interpreted with a methodology similar to the one used in studies of linear-rheology. The Zo modulus and the characteristic time constant of the process (?c) were obtained through a variation of Bode graphics. From the analysis of these parameters as a function of DNA concentration it is possible to determine the overlap (C*) and entanglement (Ce) concentrations of the system. These concentrations allow identifying conditions where interactions and entanglements between DNA molecules are present. An interpretation of the characteristic time constant values is proposed according to the structural rearrangement of the electrochemical double-layer. � The Electrochemical Society.
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