Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.12104/39180
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dc.contributor.authordel Trujillo-Murillo, K.C.
dc.contributor.authorPerez-Ibave, D.C.
dc.contributor.authorRios-Ibarra, C.P.
dc.contributor.authorRamirez-Valles, E.G.
dc.contributor.authorRincon-Sanchez, A.R.
dc.contributor.authorRivas-Estilla, A.M.
dc.date.accessioned2015-09-15T17:14:11Z-
dc.date.available2015-09-15T17:14:11Z-
dc.date.issued2011
dc.identifier.urihttp://www.scopus.com/inward/record.url?eid=2-s2.0-79960422263&partnerID=40&md5=3e6979828a212368709d11cf1bb4101e
dc.identifier.urihttp://hdl.handle.net/20.500.12104/39180-
dc.description.abstractBackground: Our aim was to develop a quantitative real-time polymerase chain reaction (qPCR) assay for the quantitation of hepatitis C virus (HCV) RNA samples from patients infected with different HCV genotypes. Methods: A standard curve was generated by amplification of serial dilutions of HCV-plasmid (pFKI389-NS3-3') harboring genotype 1b HCV-subgenomic replicon. Samples from 15 HCV-infected patients (genotypes 1, 2, and 3) were analyzed to quantify HCV-RNA by qPCR with primers and probes specific for the 5'-UTR viral region. Results: The HCV qPCR assay had a sensitivity of 100 copies/reaction with a dynamic range of detection between 10 2-20 � 10 6 HCV copies. The assay was highly reproducible with a low coefficient of variation. We observed that the HCV genotypes included could be identified by our method. Conclusions: Our results showed that this modified qPCR assay provides a valid platform for quantifying HCV-RNA, combining good analytical sensitivity with a wide dynamic range and high reproducibility.
dc.relation.isreferencedbyScopus
dc.relation.isreferencedbyWOS
dc.titleAbsolute quantitation of different genotypes of hepatitis C virus RNA in clinical samples by a modified real-time PCR method
dc.typeArticle
dc.identifier.doi10.1309/LMHXO54SA8GYPIRH
dc.relation.ispartofjournalLaboratory Medicine
dc.relation.ispartofvolume42
dc.relation.ispartofissue6
dc.relation.ispartofpage333
dc.relation.ispartofpage337
dc.subject.keywordHCV genotype; HCV-RNA; Hepatitis C virus; Nucleotides; Polymerase chain reaction; qPCR; Threshold cycle.; Untranslated region
dc.contributor.affiliationdel Trujillo-Murillo, K.C., Department of Biochemistry and Molecular Medicine, School of Medicine, Autonomous University of Nuevo Leon, Monterrey, N.L, Mexico; P�rez-Ibave, D.C., Department of Biochemistry and Molecular Medicine, School of Medicine, Autonomous University of Nuevo Leon, Monterrey, N.L, Mexico; R�os-Ibarra, C.P., Department of Biochemistry and Molecular Medicine, School of Medicine, Autonomous University of Nuevo Leon, Monterrey, N.L, Mexico; Ramirez-Valles, E.G., Department of Biochemistry and Molecular Medicine, School of Medicine, Autonomous University of Nuevo Leon, Monterrey, N.L, Mexico; Rinc�n-S�nchez, A.R., Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Jalisco, Mexico; Rivas-Estilla, A.M., Department of Biochemistry and Molecular Medicine, School of Medicine, Autonomous University of Nuevo Leon, Monterrey, N.L, Mexico
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