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|Title:||Modelling of optical absorption of silver NP's produced by UV radiation embedded in mesostructured silica films|
|Abstract:||A detailed protocol is described to induce chronic stress in BALB/c mice, which affects the hypothalamic-pituitary-adrenal (HPA) axis. The protocol is based on a combination of two mild physical stressors: restraint stress and forced swimming. Physical stressors were applied on an alternative schedule: one day restraint, next day swimming, during a 28-day period. Mice were sacrificed at days 7, 14, 21 and 28 and plasma was obtained. Optimized chromatographic system with electrochemical detection and a commercially available enzyme immunoassay kit were used to measure catecholamines [representative of the activation of the autonomic nervous system (ANS)] and corticosterone (representative of the activation of the HPA axis). Corticosterone levels increased in mice under stress and remained significantly higher in stressed mice compared to control animals throughout the experimental procedure, indicating that mice did not show habituation to the combined stress. In our experimental conditions, catecholamine levels were not useful as an index of stress. The stress model applied here provoked a steady activation of the HPA axis resulting in the constant secretion of corticosterone from the adrenal gland, which may prevent the activation of the ANS axis. The simple and economic stress model presented here affected the HPA axis but not the ANS of BALB/c mice and is useful for the study of metabolic stress-related gastric pathology and stress hormone secretion in mice. " 2012 Elsevier B.V.",,,,,,"10.1016/j.jneumeth.2012.10.015",,,"http://hdl.handle.net/20.500.12104/38931","http://www.scopus.com/inward/record.url?eid=2-s2.0-84871956946&partnerID=40&md5=32c80de8779c420abf941e955b887aed|
http://ovidsp.ovid.com/ovidweb.cgi?T=JS&CSC=Y&NEWS=N&PAGE=fulltext&D=medl&AN=23206838",,,,,,"1",,"Journal of Neuroscience Methods",,"70
MEDLINE",,,,"Index Medicus;Adrenal Glands/pp [Physiopathology];Animals;Catecholamines/bl [Blood];Chromatography, High Pressure Liquid;Chronic Disease;Corticosterone/bl [Blood];Disease Models, Animal;Electrochemistry;Female;Habituation, Psychophysiologic;Hormones/bl [Blood];Hypothalamo-Hypophyseal System/pp [Physiopathology];Immunoenzyme Techniques;Mice;Mice, Inbred BALB C;Pituitary-Adrenal System/pp [Physiopathology];Restraint, Physical;Stress, Psychological/pp [Physiopathology];Swimming/ph [Physiology];Swimming/px [Psychology]",,"BALB/c mice; Catecholamines; Corticosterone; Forced swimming stress; Habituation; Restraint stress/Biochemistry & Molecular Biology; Neurosciences & Neurology",,,,,,"A chronic combinatory stress model that activates the HPA axis and avoids habituation in BALB/C mice",,"Article" "44670","123456789/35008",,"Valverde-Aguilar, G., CICATA Unidad Legaria, IPN, Legaria 694, Col Irrigación, Miguel Hidalgo, Ciudad de México, C. P. 11500 DF, Mexico; García-Macedo, J.A., Departamento de Estado Sólido, Instituto de Física, Universidad Nacional Autónoma de México, México, DF C. P. 04510, Mexico; Rentería-Tapia, V.M., Departamento de Ciencias Naturales y Exactas, Universidad de Guadalajara, Centro Universitario de Los Valles, Ameca, JAL C. P. 46600, Mexico; Gómez, R.W., Facultad de Ciencias, Universidad Nacional Autónoma de México, Av. Universidad 3000, Copilco el Bajo, Coyoacán, México, 04510 DF, Mexico; Quintana-García, M., Facultad de Ciencias, Universidad Nacional Autónoma de México, Av. Universidad 3000, Copilco el Bajo, Coyoacán, México, 04510 DF, Mexico",,"Valverde-Aguilar, G.
Quintana-García, M.",,"2011",,"Hexagonal mesostructured films containing silver ions were obtained by sol-gel method. Brij 58 was used to produce channels into the film, which house these ions. The films were exposure to UV radiation to produced silver metallic nanoparticles. The presence of the metallic nanoparticles was determined by infrared spectroscopy and optical absorption. Besides, these nanoparticles and core-shell structures of silver-silver oxide nanoparticles were identified by high-resolution transmission electronic microscopy. From these measurements, the obtained size range for silver nanoparticles was 6.1 nm. The absorption spectrum located at 440 nm was modelled and well fitted with the Gans theory considering refractive index higher than the one coming from host matrix. This index is explained because the silver oxide shell modifies the local surrounding medium of the metallic nanoparticles. " 2011 Springer Science+Business Media B.V.
|Appears in Collections:||Producción científica UdeG|
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