Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.12104/45013
Title: The adenylyl cyclase Rv2212 modifies the proteome and infectivity of Mycobacterium bovis BCG
Author: Torres-Carrillo, N.M.
Ruiz-Noa, Y.
Martinez-Bonilla, G.E.
Leyva-Torres, S.D.
Torres-Carrillo, N.
Palafox-Sanchez, C.A.
Navarro-Hernandez, R.E.
Rangel-Villalobos, H.
Oregon-Romero, E.
Munoz-Valle, J.F.
Issue Date: 2012
Abstract: Introduction: Rheumatoid arthritis (RA) is a common autoimmune disease with a complex genetic background. The PTPN22 gene encodes lymphoid tyrosine phosphatase LYP, a potent negative regulator of T cell activation. Polymorphic variants of this gene have previously been associated with various autoimmune disorders. The +1858C/T single-nucleotide polymorphism (SNP) (rs2476601), in the exon 14 of the PTPN22 gene has been associated with susceptibility to RA in several population. Objective: The aim of this work was to investigate whether the +1858C/T of the PTPN22 gene is associated with susceptibility to RA in Western Mexico population. Methods: A total of 309 unrelated RA patients, classified according to American College of Rheumatology (ACR) 1987 criteria, as well as 347 controls residents from Western Mexico were recruited for this study. The DNA samples were genotyped for +1858C/T PTPN22 gene SNP using the PCR-RFLP technique. Antibodies to cyclic citrullinated peptides (anti-CCP) were measured by enzyme-linked immunosorbent assay (ELISA). Results: The frequency of +1858T risk allele was significantly increased in patients with RA compared with controls (p= 0.001, OR = 2.83, 95%CI = 1.50-5.32). To confirm this results we established a comparison between subjects carrying of CT. +. TT genotypes versus those carrying CC genotype, between both groups (p= 0.004, OR = 2.65, 95%CI = 1.33-5.36). Nevertheless, we not observed association of the +1858C/T PTPN22 gene SNP with clinical activity and functional disability in RA patients. Likewise, the +1858T variant in RA patients seropositive for anti-CCP antibodies, increased the risk for RA (p= 0.008, OR = 2.5, 95%CI = 1.3-5.0) when we compared with controls; however, in the group of seronegative patients, no was found significant difference (p= 0.1, OR = 2.5, 95%CI = 0.9-7.2). Conclusions: Our results support the association of the +1858T risk allele of the +1858C/T PTPN22 polymorphism with susceptibility to RA and confirm that, in combination with anti-CCP antibodies, this SNP influence the autoimmune processes towards a development of RA in Mexican population. " 2012 Elsevier B.V.",,,,,,"10.1016/j.imlet.2012.05.007",,,"http://hdl.handle.net/20.500.12104/45013","http://www.scopus.com/inward/record.url?eid=2-s2.0-84865375216&partnerID=40&md5=8ad289d7d3914674cebbaa76832094c5
http://ovidsp.ovid.com/ovidweb.cgi?T=JS&CSC=Y&NEWS=N&PAGE=fulltext&D=medl&AN=22743847",,,,,,"01-feb",,"Immunology Letters",,"41
46",,"147",,"Scopus
MEDLINE",,,,"Index Medicus;Adolescent;Adult;Aged;Aged, 80 and over;Alleles;Arthritis, Rheumatoid/di [Diagnosis];Arthritis, Rheumatoid/ge [Genetics];Autoantibodies/bl [Blood];Case-Control Studies;Female;Gene Frequency;Genetic Predisposition to Disease;Genotype;Humans;Male;Mexico;Middle Aged;Polymorphism, Single Nucleotide;Protein Tyrosine Phosphatase, Non-Receptor Type 22/ge [Genetics];Young Adult",,"Anti-CCP antibodies; Polymorphism; PTPN22 gene; Rheumatoid arthritis",,,,,,"The +1858C/T PTPN22 gene polymorphism confers genetic susceptibility to rheumatoid arthritis in Mexican population from the Western Mexico",,"Article" "46806","123456789/35008",,"Pedroza-Roldán, C., Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C., Col. Colinas de la NormalGuadalajara, Jalisco, Mexico, Dpto. de Medicina Veterinaria. Hospital Veterinario-CUCBA, Universidad de Guadalajara, Av. Prolongación Parres Arias No. 735. Col. Bosques Del Centinela II. C.P. 45187. ZapopanJalisco, Mexico; Aceves-Sánchez, M.J., Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C., Col. Colinas de la NormalGuadalajara, Jalisco, Mexico; Zaveri, A., Department of Molecular Reproduction, Development and Genetics, Indian Institute of ScienceBangalore, India; Charles-Niño, C., Centro Universitario de Ciencias de la Salud (CUCS), Universidad de Guadalajara, Sierra Mojada 950, Edificio PGuadalajara, Jalisco, Mexico; Elizondo-Quiroga, D.E., Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C., Col. Colinas de la NormalGuadalajara, Jalisco, Mexico; Hernández-Gutiérrez, R., Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C., Col. Colinas de la NormalGuadalajara, Jalisco, Mexico; Allen, K., Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C., Col. Colinas de la NormalGuadalajara, Jalisco, Mexico; Visweswariah, S.S., Department of Molecular Reproduction, Development and Genetics, Indian Institute of ScienceBangalore, India; Flores-Valdez, M.A., Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C., Col. Colinas de la NormalGuadalajara, Jalisco, Mexico",,"Pedroza-Roldan, C.
Aceves-Sanchez, M.J.
Zaveri, A.
Charles-Nino, C.
Elizondo-Quiroga, D.E.
Hernandez-Gutierrez, R.
Allen, K.
Visweswariah, S.S.
Flores-Valdez, M.A.",,"2014",,"All organisms have the capacity to sense and respond to environmental changes. These signals often involve the use of second messengers such as cyclic adenosine monophosphate (cAMP). This second messenger is widely distributed among organisms and coordinates gene expression related with pathogenesis, virulence, and environmental adaptation. Genomic analysis in Mycobacterium tuberculosis has identified 16 adenylyl cyclases (AC) and one phosphodiesterase, which produce and degrade cAMP, respectively. To date, ten AC have been biochemically characterized and only one (Rv0386) has been found to be important during murine infection with M. tuberculosis. Here, we investigated the impact of hsp60-driven Rv2212 gene expression in Mycobacterium bovis Bacillus Calmette-Guerin (BCG) during growth in vitro, and during macrophage and mice infection. We found that hsp60-driven expression of Rv2212 resulted in an increased capacity of replication in murine macrophages but an attenuated phenotype in lungs and spleen when administered intravenously in mice. Furthermore, this strain displayed an altered proteome mainly affecting proteins associated with stress conditions (bfrB, groEL-2, DnaK) that could contribute to the attenuated phenotype observed in mice. " 2014, Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i.
URI: http://hdl.handle.net/20.500.12104/45027
http://www.scopus.com/inward/record.url?eid=2-s2.0-84919335827&partnerID=40&md5=92c4ab10320534a42ef4122f6231e511
Appears in Collections:Producción científica UdeG

Files in This Item:
There are no files associated with this item.


Items in RIUdeG are protected by copyright, with all rights reserved, unless otherwise indicated.