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|Title:||Quinolizidine alkaloids isolated from Lupinus species enhance insulin secretion|
|Author:||Martinez- Espinosa, J.C.|
|Abstract:||The use of Raman spectroscopy to analyze blood biochemistry and hence distinguish between normal and abnormal blood was investigated. The blood samples were obtained from 6 patients who were clinically diagnosed with leukemia and 6 healthy volunteer. The imprint was put under the microscope and several points were chosen for Raman measurement. All spectra were collected at confocal Raman micro-spectroscopy (Renishaw) with NIR 830 nm laser. It is shown that the serum samples from, patients with leukemia and from the control group can be discriminated when the multivariate statistical methods of principal component analysis (PCA) and linear discriminated analysis (LDA) is applied to their Raman spectra. The ratios of some band intensities were analyzed and some band ratios were significant and corresponded to proteins, phospholipids, and polysaccharides. In addition, currently the degree of damage to the bone marrow is estimated through biopsies and therefore it is a very procedure painful. The preliminary results suggest that Raman spectroscopy could be a new technique to study the bone marrow using just blood samples. " 2008 American Institute of Physics.",,,,,,"10.1063/1.2979283",,,"http://hdl.handle.net/20.500.12104/44052","http://www.scopus.com/inward/record.url?eid=2-s2.0-52649141520&partnerID=40&md5=074d4dab038ce270fd483ed6ddd02f86",,,,,,,,"AIP Conference Proceedings",,"252|
254",,"1032",,"Scopus",,,,,,"Bone; Leukemia; Marrow; Multivariate; Raman; Serum; Spectroscopy",,,,,,"Raman spectroscopy: A new proposal for the detection of leukemia using blood samples",,"Conference Paper" "45814","123456789/35008",,"García López, P.M., Laboratory of Biotechnology, Department of Botany and Zoology, CUCBA, United States, United States; De La Mora, P.G., Inst. Chron.-Degenerative Dis. P., CUCS, University of Guadalajara, Mexico, Mexico; Wysocka, W., Laboratory of Stereochemistry, Faculty of Chemistry, Adam Mickiewicz Univ., Poznan, P., Poland; Maiztegui, B., CENEXA - Ctr. Exp. Appl. E., PAHO/WHO Collaborating Centre), Sch. Med., Fac. Cie. Medicas, C., Argentina; Alzugaray, M.E., CENEXA - Ctr. Exp. Appl. E., PAHO/WHO Collaborating Centre), Sch. Med., Fac. Cie. Medicas, C., Argentina; Del Zotto, H., CENEXA - Ctr. Exp. Appl. E., PAHO/WHO Collaborating Centre), Sch. Med., Fac. Cie. Medicas, C., Argentina; Borelli, M.I., CENEXA - Ctr. Exp. Appl. E., PAHO/WHO Collaborating Centre), Sch. Med., Fac. Cie. Medicas, C., Argentina",,"García Lopez, P.M.
De La Mora, P.G.
Del Zotto, H.
Borelli, M.I.",,"2004",,"We have analyzed the effect of quinolizidine alkaloids from Lupinus species upon insulin secretion. Isolated normal rat islets were incubated with 3.3, 8.3, and 16.7 mM glucose, in the presence or absence of different concentrations of lupanine (0.05, 0.5, and 1.0 mM), 13-?-OH lupanine, 17-oxo-lupanine, and 2-thionosparteine. Insulin release was measured by radioimmunoassay. While 2-thionosparteine enhanced insulin secretion at all glucose concentrations, lupanine did at 8.3 and 16.7 mM, and 13-?-OH lupanine or 17-oxo-lupanine only at 16.7 mM glucose. Diazoxide (0.1 mM) decreased the effect of all alkaloids, without suppressing it completely. Consequently, blockage of ?-cell K ATP-sensitive channels is at least one of the mechanisms involved in the enhancing secretagogue effects of quinolizidine alkaloids. The fact that 13-?-OH lupanine and 17-oxo-lupanine only exert their secretagogue effect at high glucose concentrations could be of additional value when considering their potential use in the treatment of type 2 diabetes. " 2004 Elsevier B.V. All rights reserved.
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