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|Title:||Prevalence of Salmonella in chorizo and its survival under different storage temperatures|
|Abstract:||The prevalence of Salmonella in chorizo, a raw pork sausage, was studied in two locations in Mexico. Sixty samples were collected from butcher shops and retail markets in Guadalajara city, and another 80 samples were collected from butcher shops (40 samples) and grocery stores (40 samples) in Queretaro city. Samples obtained from butcher shops had been produced at the same butchery, whereas those obtained from grocery stores in Queretaro had been processed at national meat processing plants. In Guadalajara, 88% of the samples tested positive to Salmonella. In Queretaro, 78% of the samples collected from butcher shops and 5% of the samples collected from grocery stores tested positive to Salmonella. The ability to isolate Salmonella from chorizo was affected by the method of analysis and was best achieved by preenrichment of 20 g of sample in buffered peptone water for 24 h, followed by enrichment in tetrathionate brilliant green and selenite cystine broths. The five most frequent serovars isolated were S. derby (26%), S. anatum (14%), S. infantis (14%), S. typhimurium (12%) and S. brandenburg (6%). Five selected samples that tested positive for Salmonella were selected for enumeration by most probable number (MPN) and subsequent survival studies during storage of chorizo at refrigeration (4-6°C) and at room temperature (18-22°C). Initial numbers of Salmonella varied from 900 to 0.04 MPN g-1, and with the exception of the sample containing 900 Salmonella MPN g-1, all other samples were cleared from this pathogen after 21 days of refrigeration or 5-10 days at room temperature. Salmonella contamination in chorizo can be prevented by improving the bacteriological quality and safety of the raw materials and by controlling the natural aging process of this product.|
|Appears in Collections:||Producción científica UdeG|
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