Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.12104/41605
Title: Nutritional support with nucleotide addition favors immune response in severely malnourished infants
Author: Galvan-Ramirez, M.L.
Madriz Elisondo, A.L.
Torres, C.P.R.
Luna-Pasten, H.
Perez, L.R.R.
Rincon-Sanchez, A.R.
Franco, R.
Salazar-Montes, A.
Correa, D.
Issue Date: 2010
Abstract: Toxoplasmosis is an infection caused by Toxoplasma gondii, an intracellular obligate parasite. Its transmission has usually been attributed to ingestion of undercooked or raw meat. The frequency of T. gondii in pork, the most common meat for human consumption in Jalisco, Mexico, is unknown; in Guadalajara city high prevalence of human toxoplasmosis has been documented. Forty-eight samples of pork meat from butcher shops in Ocotlan city were analyzed. Through bioassay, 50 g of tissue was homogenized in an acidic pepsin solution and inoculated subcutaneously to previously immunosuppressed mice. Blood samples from the mice tail vein were obtained before inoculation and 7, 14, 28, and 45 days postinoculation to analyze anti-Toxoplasma immunoglobulin (Ig) M and IgG antibody kinetics by indirect enzyme-linked immunosorbent assay. For histopathology, small fragments of the brain, lungs, heart, and skeletal muscle were extracted on day 45 and were stained with hematoxylin and eosin. Also, DNA was extracted from the pork meat for PCR amplification of the Bl gene. Even though all pork samples were negative by histopathology and PCR, IgG and IgM antibodies against T. gondii were detected in 1 of the 48 inoculated mice, reflecting a frequency of 2.1% positive pork meat, which is lower than expected but similar to that found in other regions. Copyright " International Association for Food Protection.",,,,,,,,,"http://hdl.handle.net/20.500.12104/41605","http://www.scopus.com/inward/record.url?eid=2-s2.0-77954643034&partnerID=40&md5=761e63533919b8f3600716cbdc39a866
http://ovidsp.ovid.com/ovidweb.cgi?T=JS&CSC=Y&NEWS=N&PAGE=fulltext&D=med5&AN=20537271",,,,,,"6",,"Journal of Food Protection",,"1121
1123",,"73",,"Scopus
MEDLINE
WOS",,,,"Index Medicus;Animals;Antibodies, Protozoan/bl [Blood];Biological Assay;Food Contamination/an [Analysis];Food Parasitology;Humans;Immunoglobulin G/bl [Blood];Immunoglobulin M/bl [Blood];Meat/ps [Parasitology];Mexico/ep [Epidemiology];Mice;Prevalence;Swine;Toxoplasma/im [Immunology];Toxoplasma/ip [Isolation & Purification];Toxoplasmosis/pc [Prevention & Control];Toxoplasmosis/tm [Transmission];Toxoplasmosis, Animal/im [Immunology];Toxoplasmosis, Animal/ps [Parasitology];Toxoplasmosis, Animal/pa [Pathology]",,,,,,,,"Frequency of Toxoplasma gondii in Pork Meat in Ocotlan, Jalisco, Mexico",,"Article" "45049","123456789/35008",,"Vásquez-Garibay, E., Instituto de Nutrición Humana, Depto. de Reproducción Humana, Crecimiento Y Desarrollo Infantil, Mexico, Depto. Reprod. Humana, C., Instituto de Nutrición Humana, Hosp. Civ. Guadalajara Dr. Juan I., Salvador Quevedo y Zubieta #750, S. L., 44340, Guadalajara, Jalisco, Mexico; Méndez-Estrada, C., U. de Estud. de Nutr. Infantil, División de Pediatría, Hosp. Civ. Guadalajara Dr. Juan I., Jalisco, Mexico; Romero-Velarde, E., Instituto de Nutrición Humana, Depto. de Reproducción Humana, Crecimiento Y Desarrollo Infantil, Mexico; García-Iglesias, M.T., Laboratorio de Inmunología, Ctro. Univ. de Ciencias de la Salud, Universidad de Guadalajara, Jalisco, Mexico; Campollo-Rivas, O., Instituto de Nutrición Humana, Depto. de Reproducción Humana, Crecimiento Y Desarrollo Infantil, Mexico",,"Vasquez-Garibay, E.
Mendez-Estrada, C.
Romero-Velarde, E.
García-Iglesias, M.T.
Campollo-Rivas, O.",,"2004",,"Background Adequate nutritional support of severe primary protein energy malnutrition (PEM) is indispensable for achieving normal immune response. However, an ideal dietetic criterion has not yet been established or accepted by the majority of health professionals caring for malnourished infants. Our objective was to demonstrate improvement in immune response in infants with PEM who received starting infant formula with nucleotides. Methods Twelve malnourished infants 3-18 months of age were included in our study and were fed through a nasogastric tube with infant formula (3.35 kJ/mL) for 2 weeks and ad libitum for an additional 2 weeks. Anthropometric measurements and immunologic indicators such as phagocytosis, microbicidal activity, chemotaxis, and cell proliferation index were determined. The sample was divided into two groups; group 1 (n=6) was fed formula with nucleotides added, while group 2 (n=6) was fed a formula with no nucleotides. Paired t test was used to determine differences between initial and final results for each group for comparison between group 1 vs. group 2; a nonparametric Mann-Whitney U test was used for immunologic indicators. Results Comparison of immunologic indicators showed no significant difference between groups. Groups 1 and 2 showed improvement in phagocytosis and microbicidal activity (p <0.05) and group 2 additionally showed improvement in cell proliferation index. Conclusions Infant formula with intake of 837 kJ/kg/d (200 kcal/kg/d) and proteins of 4 g/kg/d in infants with PEM had a favorable impact on immunologic indicators regardless of addition of nucleotides. " 2004 IMSS. Published by Elsevier Inc.
URI: http://hdl.handle.net/20.500.12104/43270
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