Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.12104/40774
Title: DNA transitions by an adsorption impedance study
Author: Bravo-Anaya, L.M.
Macias, E.R.
Ramos, F.C.
Fernandez, V.V.A.
Casillas, N.
Soltero, J.F.A.
Larios-Duran, E.R.
Issue Date: 2012
Abstract: We present an EIS study and its analysis to determine the conformational behavior of DNA calf-thymus (13 kbp). The study is carried out at open circuit potential at a pH of 7.3. The results are interpreted in terms of the adsorption impedance theory by using an equivalent circuit approach similar to the one proposed by Frumkin-Melik-Gaikazyan-Randles. All equivalent circuit parameters are inspected as a function of DNA concentrations where it is possible to identify two transitions on the double-layer structure at DNA ca. 0.33 0.07 and 1.50 mg/mL related to the overlap, C, and entanglement, Ce, concentrations. " 2012 The Electrochemical Society.",,,,,,"10.1149/2.014202eel",,,"http://hdl.handle.net/20.500.12104/40774","http://www.scopus.com/inward/record.url?eid=2-s2.0-84875693651&partnerID=40&md5=1e4373dae0d29882fa5bf3a263cd27e2",,,,,,"2",,"ECS Electrochemistry Letters",,"G1
G3",,"1",,"Scopus
WOS",,,,,,,,,,,,"DNA conformational transitions at different concentrations and temperatures monitored by EIS",,"Article" "42554","123456789/35008",,"Urbina-Cano, P., Laboratorio de Citogenética Genotoxicidad y Biomonitoreo, Instituto de Genética Humana Dr. Enriquo Corona Rivera, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico; Bobadilla-Morales, L., Laboratorio de Citogenética Genotoxicidad y Biomonitoreo, Instituto de Genética Humana Dr. Enriquo Corona Rivera, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico; Ramírez-Herrera, M.A., Laboratorio de Neurofisiologia, Departamento de Fisiología, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico; Corona-Rivera, J.R., Laboratorio de Citogenética Genotoxicidad y Biomonitoreo, Instituto de Genética Humana Dr. Enriquo Corona Rivera, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico; Mendoza-Magaña, M.L., Laboratorio de Neurofisiologia, Departamento de Fisiología, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico; Troyo-Sanromán, R., Laboratorio de Citogenética Genotoxicidad y Biomonitoreo, Instituto de Genética Humana Dr. Enriquo Corona Rivera, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico; Corona-Rivera, A., Laboratorio de Citogenética Genotoxicidad y Biomonitoreo, Instituto de Genética Humana Dr. Enriquo Corona Rivera, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico, Unidad de Citogenética, OPD Hospital Civil Fray Antonio Alcalde, Guadalajara, Jalisco, Mexico, Laboratorio de Citogenética Genotoxicidad y Biomonitoreo, Institute de Genética Humana Dr. Enrique Corona Rivera, Universidad de Guadalajara, Sierra Mojada # 950, S.L., CP-44340, Guadalajara, Jalisco, Mexico",,"Urbina-Cano, P.
Bobadilla-Morales, L.
Ramirez-Herrera, M.A.
Corona-Rivera, J.R.
Mendoza-Magana, M.L.
Troyo-Sanroman, R.
Corona-Rivera, A.",,"2006",,"Dietary polyphenolics. such as curcumin. have shown antioxidant and anti-inflammatory effects. Some antioxidants cause DNA strand breaks in excess of transition metal ions, such as copper. The aim of this study was to evaluate the in vitro effect of curcumin in the presence of increasing concentrations of copper to induce DNA damage in murine leukocytes by the comet assay. Balb-C mouse lymphocytes were exposed to 50 ?M curcumin and various concentrations of copper (10 ?M, 100 ?M and 200 ?M). Cellular DNA damage was detected by means of the alkaline comet assay. Our results show that 50 ?M curcumin in the presence of 100-200 ?M copper induced DNA damage in murine lymphocytes. Curcumin did not inhibit the oxidative DNA damage caused by 50 ?M H 2O2 in mouse lymphocytes. Moreover, 50 ?M curcumin alone was capable of inducing DNA strand breaks under the tested conditions. The increased DNA damage by 50 ?M curcumin was observed in the presence of various concentrations of copper, as detected by the alkaline comet assay.",,,,,,,,,"http://hdl.handle.net/20.500.12104/40775","http://www.scopus.com/inward/record.url?eid=2-s2.0-33845455536&partnerID=40&md5=17153ee7de5ad4143db4924e972871a1
http://ovidsp.ovid.com/ovidweb.cgi?T=JS&CSC=Y&NEWS=N&PAGE=fulltext&D=med5&AN=17132903",,,,,,"4",,"Journal of Applied Genetics",,"377
382",,"47",,"Scopus
WOS
MEDLINE",,,,"Index Medicus;Animals;Comet Assay;Copper/to [Toxicity];Curcumin/to [Toxicity];DNA Damage;Dose-Response Relationship, Drug;Female;Lymphocytes/de [Drug Effects];Lymphocytes/me [Metabolism];Mice;Mice, Inbred BALB C",,"Anti-oxidant; Comet assay; Copper; Curcumin; DNA strand breaks; Hydrogen peroxide; Pro-oxidant",,,,,,"DNA damage in mouse lymphocytes exposed to curcumin and copper",,"Review" "42555","123456789/35008",,"Mesa-Cornejo, V.M., Departamento de Genética Humana, Universidad de Guadalajara, Guadalajara, Jal., Mexico; Barros-Núñez, P., Departamento de Genética Humana, Universidad de Guadalajara, Guadalajara, Jal., Mexico, División de Genética, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Guadalajara, Jal., Mexico; Medina-Lozano, C., Departamento de Genética Humana, Universidad de Guadalajara, Guadalajara, Jal., Mexico, División de Genética, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Guadalajara, Jal., Mexico, Sierra Mojada 800. Col. Independencia, CP 44340. Guadalajara, Jalisco, Mexico",,"Mesa-Cornejo, V.M.
Barros-Nunez, P.
Medina-Lozano, C.",,"2006",,"[No abstract available]",,,,,,,,,"http://hdl.handle.net/20.500.12104/40776","http://www.scopus.com/inward/record.url?eid=2-s2.0-33745044996&partnerID=40&md5=7512663fbe3a3f8a74d828d9559a5a46",,,,,,"1",,"Gaceta Medica de Mexico",,"81
82",,"142",,"Scopus",,,,,,,,,,,,"DNA methylation: Diagnostic and prognostic marker of cancer [MetilaciZapotitlánn del ADN: Marcador diagnóstico y pronZapotitlánstico de cáncer]",,"Short Survey" "42556","123456789/35008",,"Bravo-Anaya, L.M., Universidad de Guadalajara, Departamento de Ingeniería Química, C. P. 44430 Guadalajara, Jalisco, Mexico; Macías, E.R., Universidad de Guadalajara, Departamento de Ingeniería Química, C. P. 44430 Guadalajara, Jalisco, Mexico; Ramos, F.C., Centro Universitario UTEG, Departamento de Investigación, C. P. 44460 Guadalajara, Jalisco, Mexico; Álvarez-Ramírez, J.G., Universidad Juárez Autónoma de Tabasco, División Académica de Ingeniería y Arquitectura, C. P. 86040 Cunduacán, Tabasco, Mexico; Casillas, N., Universidad de Guadalajara, Departamento de Química, C. P. 44430 Guadalajara, Jalisco, Mexico; Soltero, J.F.A., Universidad de Guadalajara, Departamento de Ingeniería Química, C. P. 44430 Guadalajara, Jalisco, Mexico; Larios-Durán, E.R., Universidad de Guadalajara, Departamento de Ingeniería Química, C. P. 44430 Guadalajara, Jalisco, Mexico",,"Bravo-Anaya, L.M.
Macias, E.R.
Ramos, F.C.
Alvarez-Ramirez, J.G.
Casillas, N.
Soltero, J.F.A.
Larios-Duran, E.R.",,"2013",,"We present a detailed adsorption study of the interface platinum-calf thymus DNA(13 kbp) as a function of concentration, temperature and a constant pH of 7.3. The application of EIS technique and its analysis by complex capacitance spectra is used to determine the structural behavior of the system. The study was carried out at open circuit potential and three different temperatures, 20, 30 and 40°C, making possible to detect changes in the double-layer structure at the electrode interface. An equivalent circuit approach similar to the one proposed by Frumkin-Melik-Gaikazyan-Randles is used to analyze the results and to determine a characteristic time-constant of the adsorption process. The equivalent circuit parameters, i.e. double layer capacitance (Cdl), adsorption capacitance (Cad), adsorption resistance (Rad) and charge transfer resistance (R ct) are inspected as a function of DNA concentrations and temperatures. From this analysis as well as the study of the characteristic time-constant it was possible to identify two transitions of the double-layer structure atDNA concentrations ca. 0.36 ± 0.07 and 1.50 mg/mL. EIS results transformed into complex capacitance spectra are successfully compared with those obtained by rheological methods which allow us to identify molecule interactions at the overlap and entanglement concentrations, C* and C e, respectively. " 2013 The Electrochemical Society.
URI: http://hdl.handle.net/20.500.12104/40777
http://www.scopus.com/inward/record.url?eid=2-s2.0-84875749989&partnerID=40&md5=b719accc5d90c65fe15e4b9843a6bf1b
Appears in Collections:Producción científica UdeG

Files in This Item:
There are no files associated with this item.


Items in RIUdeG are protected by copyright, with all rights reserved, unless otherwise indicated.