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Title: Development and validation of a High Performance Liquid Chromatography-Diode Array Detection method for the determination of aging markers in tequila
Author: Martinez-Espinosa, J.C.
Gonzalez-Solis, J.L.
Miranda-Beltran, M.L.
Soria-Fregoso, C.
Medina-Valtierra, J.
Frausto-Reyes, C.
Issue Date: 2009
Abstract: The use of Raman spectroscopy to analyze blood biochemistry and hence distinguish between normal and abnormal blood was investigated. Blood samples were obtained from 6 patients who were clinically diagnosed with leukemia and 6 healthy volunteers. The imprint was put under the microscope and several points were chosen for Raman measurement. All the spectra were collected by a confocal Raman micro-spectroscopy (Renishaw) with a NIR 830 nm laser. It is shown that the serum samples from patients with leukemia and from the control group can be discriminated when the multivariate statistical methods of principal component analysis (PCA) and linear discriminated analysis (LDA) are applied to their Raman spectra. The ratios of some band intensities were analyzed and some band ratios were significant and corresponded to proteins, phospholipids, and polysaccharides. The preliminary results suggest that Raman Spectroscopy could be a new technique to study the degree of damage to the bone marrow using just blood samples instead of biopsies, treatment very painful for patients. " 2009 American Institute of Physics.",,,,,,"10.1063/1.3175637",,,"","",,,,,,,,"AIP Conference Proceedings",,"99
103",,"1142",,"Scopus",,,,,,"Bone marrow; Leukemia; Multivariate; Raman spectroscopy; Serum",,,,,,"Detection of leukemia with blood samples using raman spectroscopy and multivariate analysis",,"Conference Paper" "42371","123456789/35008",,"Muñoz-Muñoz, A.C., Centro de Investigacion y Asistencia en Tecnologia y Diseno del Estado de Jalisco, Av. Normalistas 800, 44270 Guadalajara, Jalisco, Mexico; Grenier, A.C., Peace Corps México, Av. Universidad 202 Ote, 76020 Queretaro Queretaro,, Mexico; Gutiérrez-Pulido, H., Centro Universitario de Ciencias Exactas e Ingenierías, Departamento de Matemáticas, Universidad de Guadalajara, Corregidora 500, 44840 Guadalajara, Jalisco, Mexico; Cervantes-Martínez, J., Centro de Investigacion y Asistencia en Tecnologia y Diseno del Estado de Jalisco, Av. Normalistas 800, 44270 Guadalajara, Jalisco, Mexico",,"Munoz-Munoz, A.C.
Grenier, A.C.
Gutierrez-Pulido, H.
Cervantes-Martinez, J.",,"2008",,"A solid-phase extraction procedure followed by analysis by high performance liquid chromatography (HPLC) with UV-vis photodiode array detection (DAD) is proposed to simultaneously determine 11 aging markers in tequila. The method showed good intraday (n = 5) and interday (n = 3) precision, RSD < 1.6% in both cases, for each of the identified compounds. The calibration curves were linear at the tested ranges (R2 > 0.999). Good recoveries (84.2-108.5%) were obtained for 10 of the 11 compounds studied; and the LOD and LOQ ranged from 0.62 to 4.09 ?g/mL and 1.9-12.4 ?g/mL, respectively. The proposed methodology was applied to a set of 15 authentic tequila samples grouped by aging state (blanco, reposado and añejo). An ANOVA analysis combined with discriminant analysis with stepwise backward variable selection was used to differentiate between the various aging groups based on their oak related compounds content. " 2008 Elsevier B.V. All rights reserved.
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