Please use this identifier to cite or link to this item: https://hdl.handle.net/20.500.12104/40002
Title: Characterization of Enterobacteriaceae Isolates Obtained from a Tertiary Care Hospital in Mexico, Which Produces Extended-Spectrum ?-Lactamase
Author: Orozco-Guareno, E.
Almaraz, A.N.C.
Reyes, G.I.
Lopez-Ureta, L.C.
Gonzalez-Alvarez, A.
Issue Date: 2006
Abstract: The thermal behavior of hydrogels synthesized by solution polymerization between acrylamide, acrylic acid and diglycidyl acrylate (DGA) as a crosslinking agent was investigated. The structure of the hydrogel can be tightly controlled with the reaction temperature. This method produces a new type of hydrogels, which exhibit well defined structures at various scales of length simultaneously. These multi-structured hydrogels are hydrophilic, elastic, water insoluble, and soft polymers with an anisotropic optical response. The structure was observed by scanning electron microscopy (SEM), polarized light microscopy (PLM) and macroscopic visualization (CCD camera). In addition, structural transitions in the hydrogels were monitored by temperature modulated differential scanning calorimetry (TMDSC). Severe heating tests in an adiabatic oven were performed to analyze decomposition of the material. Fourier transform infrared (FTIR) spectroscopy was used to qualitatively analyze the hydrogels samples exposed to a sudden thermal treatment. " 2006 Springer-Verlag.",,,,,,"10.1007/s10973-005-7449-2",,,"http://hdl.handle.net/20.500.12104/40002","http://www.scopus.com/inward/record.url?eid=2-s2.0-33750033015&partnerID=40&md5=365b7afcc2cb2b00df95c29422d13675",,,,,,"2",,"Journal of Thermal Analysis and Calorimetry",,"511
516",,"86",,"Scopus
WOS",,,,,,"Acrylamide; Acrylic acid; Polymeric hydrogel; Structural transition; TMDSC",,,,,,"Characterization of structured acrylamide/acrylic acid hydrogels by TMDSC and microscopy",,"Article" "41772","123456789/35008",,"Morfín-Otero, R., Hospital Civil de Guadalajara, Fray Antonio Alcalde and Instituto de Patología Infecciosa y Experimental, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Mexico; Mendoza-Olazarán, S., Servicio de Gastroenterología, Hospital Universitario Dr. José Eleuterio González, Universidad Autónoma de Nuevo León, Avenida Francisco I. Madero s/n Colonia Mitras Centro, Monterrey, Nuevo Leon 64460, Mexico; Silva-Sánchez, J., Departamento de Diagnóstico Epidemiológico, Instituto Nacional de Salud Pública (CISEI), Cuernavaca, Mexico; Rodríguez-Noriega, E., Hospital Civil de Guadalajara, Fray Antonio Alcalde and Instituto de Patología Infecciosa y Experimental, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Mexico; Laca-Díaz, J., Departamento de Patología Clínica, Hospital Universitario Dr. José Eleuterio González, Universidad Autónoma de Nuevo León, Monterrey, Mexico; Tinoco-Carrillo, P., Departamento de Diagnóstico Epidemiológico, Instituto Nacional de Salud Pública (CISEI), Cuernavaca, Mexico; Petersen, L., Hospital Civil de Guadalajara, Fray Antonio Alcalde and Instituto de Patología Infecciosa y Experimental, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Mexico; López, P., Departamento de Microbiología, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey, Mexico; Reyna-Flores, F., Departamento de Diagnóstico Epidemiológico, Instituto Nacional de Salud Pública (CISEI), Cuernavaca, Mexico; Alcantar-Curiel, D., Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, Mexico; Garza-Ramos, U., Departamento de Diagnóstico Epidemiológico, Instituto Nacional de Salud Pública (CISEI), Cuernavaca, Mexico; Garza-González, E., Servicio de Gastroenterología, Hospital Universitario Dr. José Eleuterio González, Universidad Autónoma de Nuevo León, Avenida Francisco I. Madero s/n Colonia Mitras Centro, Monterrey, Nuevo Leon 64460, Mexico, Departamento de Patología Clínica, Hospital Universitario Dr. José Eleuterio González, Universidad Autónoma de Nuevo León, Monterrey, Mexico",,"Morfin-Otero, R.
Mendoza-Olazaran, S.
Silva-Sanchez, J.
Rodriguez-Noriega, E.
Laca-Diaz, J.
Tinoco-Carrillo, P.
Petersen, L.
Lopez, P.
Reyna-Flores, F.
Alcantar-Curiel, D.
Garza-Ramos, U.
Garza-Gonzalez, E.",,"2013",,"The prevalence and genetic characteristics of Escherichia coli and Klebsiella pneumoniae clinical isolates producing extended-spectrum ?-lactamase (ESBL) were examined. Between October 2010 and March 2011, E. coli (n=460) and K. pneumoniae (n=78) isolates were collected at a tertiary care hospital in Guadalajara, Mexico. The minimum inhibitory concentration (MIC) for each isolate was determined using a broth microdilution method, and ESBL production was assayed. The presence of ?-lactamase genes, bla SHV, blaCTX-M, and blaTLA-1, was detected by PCR and confirmed with sequencing. Only ESBL-producing isolates were further subjected to pulsed-field gel electrophoresis (PFGE) and plasmid profiling. All of the ESBL isolates were multidrug resistant and 75/460 (16.3%) E. coli isolates and 21/78 (26.9%) K. pneumoniae isolates were found to produce ESBL. For the E. coli isolates, >95% susceptibility to amikacin, meropenem, fosfomycin, imipenem, and nitrofurantoin was observed. For K. pneumoniae, similar results were obtained, with discrepancies observed for gentamicin and nitrofurantoin. PFGE further identified eleven pulsotypes for E. coli and three clusters of K. pneumoniae. CTX-M-15 was detected in 85% of ESBL-producing E. coli and in 76% of ESBL-producing K. pneumoniae. In contrast, SHV-5 ESBL was identified in 17% of E. coli isolates and in 86% of K. pneumoniae isolates. The bla-TLA-1 gene was not detected in any of the 96 isolates analyzed. Overall, CTX-M-15 and SHV-5 were found to have a high rate of spread throughout the hospital and were associated with strong multidrug resistance. " 2013 Mary Ann Liebert, Inc.
URI: http://hdl.handle.net/20.500.12104/39993
http://www.scopus.com/inward/record.url?eid=2-s2.0-84884561743&partnerID=40&md5=08f1898300616974db90e57fd6754cf8
http://ovidsp.ovid.com/ovidweb.cgi?T=JS&CSC=Y&NEWS=N&PAGE=fulltext&D=medl&AN=23725513
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